Experimental Validation of Bacillus anthracis A16R Proteogenomics

doi:10.1038/srep14608

	Experimental Validation of Bacillus anthracis A16R Proteogenomics
	Gao, Zhiqi 1; Wang, Zhiqiang 2,3,4; Zhang, Kun 5; Li, Yanchang 2; Zhang, Tao 2; Wang, Dongshu 1; Liu, Xiankai 1; Feng, Erling 1; Chang, Lei 2; Xu, Junjie 1; He, Simin 5; Xu, Ping 2,3,4; Zhu, Li 1; Wang, Hengliang 1
	2015-10-01
发表期刊	SCIENTIFIC REPORTS
ISSN	2045-2322
卷号	5 页码:10
摘要	Anthrax, caused by the pathogenic bacterium Bacillus anthracis, is a zoonosis that causes serious disease and is of significant concern as a biological warfare agent. Validating annotated genes and reannotating misannotated genes are important to understand its biology and mechanisms of pathogenicity. Proteomics studies are, to date, the best method for verifying and improving current annotations. To this end, the proteome of B. anthracis A16R was analyzed via one-dimensional gel electrophoresis followed by liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS). In total, we identified 3,712 proteins, including many regulatory and key functional proteins at relatively low abundance, representing the most complete proteome of B. anthracis to date. Interestingly, eight sequencing errors were detected by proteogenomic analysis and corrected by resequencing. More importantly, three unannotated peptide fragments were identified in this study and validated by synthetic peptide mass spectrum mapping and green fluorescent protein fusion experiments. These data not only give a more comprehensive understanding of B. anthracis A16R but also demonstrate the power of proteomics to improve genome annotations and determine true translational elements.
DOI	10.1038/srep14608
收录类别	SCI
语种	英语
资助项目	Key Project of China Programs for Communicable Diseases[2012ZX10004215-001-002] ; Key Project of China Programs for Drug Development[2013ZX09304101] ; National Natural Science Foundation of China[81125012] ; National Natural Science Foundation of China[81271785] ; National Natural Science Foundation of China[81471912]
WOS研究方向	Science & Technology - Other Topics
WOS类目	Multidisciplinary Sciences
WOS记录号	WOS:000362082600001
出版者	NATURE PUBLISHING GROUP
引用统计	被引频次：6[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://119.78.100.204/handle/2XEOYT63/9302
专题	中国科学院计算技术研究所期刊论文_英文
通讯作者	Xu, Ping
作者单位	1.Beijing Inst Biotechnol, State Key Lab Pathogen & Biosecur, Beijing 100071, Peoples R China 2.Beijing Inst Radiat Med, Beijing Proteome Res Ctr, Natl Engn Res Ctr Prot Drugs, State Key Lab Prote,Natl Ctr Prot Sci, Beijing 100850, Peoples R China 3.Wuhan Univ, Minist Educ, Key Lab Combinatorial Biosynth & Drug Discovery, Wuhan 430071, Peoples R China 4.Wuhan Univ, Sch Pharmaceut Sci, Wuhan 430071, Peoples R China 5.Chinese Acad Sci, Inst Comp Technol, Beijing 100190, Peoples R China
推荐引用方式 GB/T 7714	Gao, Zhiqi,Wang, Zhiqiang,Zhang, Kun,et al. Experimental Validation of Bacillus anthracis A16R Proteogenomics[J]. SCIENTIFIC REPORTS,2015,5:10.
APA	Gao, Zhiqi.,Wang, Zhiqiang.,Zhang, Kun.,Li, Yanchang.,Zhang, Tao.,...&Wang, Hengliang.(2015).Experimental Validation of Bacillus anthracis A16R Proteogenomics.SCIENTIFIC REPORTS,5,10.
MLA	Gao, Zhiqi,et al."Experimental Validation of Bacillus anthracis A16R Proteogenomics".SCIENTIFIC REPORTS 5(2015):10.