Label-free detection of macrophage polarization via specific membrane capacitance using high-throughput microfluidic impedance cytometry

doi:10.1016/j.snb.2025.139191

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	Label-free detection of macrophage polarization via specific membrane capacitance using high-throughput microfluidic impedance cytometry
	Ye, Yifei 1,2; Geng, Xiaokun 3; Kong, Qi 3; Li, Xue 4; Zhi, Tian 5; Zhang, Lingqian 1; Li, Mingxiao 1; Huang, Chengjun 1; Zhao, Yang 1; Zhao, Haiping 4
	2026-02-15
发表期刊	SENSORS AND ACTUATORS B-CHEMICAL
卷号	449 页码:11
摘要	Macrophages are highly plastic immune cells that adopt diverse functional states to modulate inflammation. Capturing this dynamic heterogeneity requires single-cell resolution. However, conventional fluorescence flow cytometry relies on multistep antibody labeling and lacks a direct quantitative read-out of cell-intrinsic properties. In this study, we identify specific membrane capacitance (Csm), an intrinsic electrical property of individual cells, as a sensitive and quantitative marker of macrophage polarization. Measured at single-cell resolution using high-throughput, label-free microfluidic impedance cytometry, Csm increases in pro-inflammatory states and decreases in anti-inflammatory conditions, offering a direct readout of functional macrophage states. Leveraging this metric, we establish a rapid drug screening platform targeting macrophage polarization. Notably, screening the histone deacetylase (HDAC) family reveals that the inhibition of Class IIa HDACs or the activation of Class III HDACs effectively suppresses pro-inflammatory polarization. These effects are validated using blood samples from stroke patients and further supported by improvement of ischemic outcomes in mouse cerebral ischemic models. Collectively, our results highlight Csm as a robust, label-free marker for real-time single-cell analysis of macrophage polarization, offering a valuable tool for both basic research and therapeutic development in neuroinflammation.
关键词	Specific membrane capacitance Microfluidic impedance cytometry Macrophage polarization Drug screening Stroke
DOI	10.1016/j.snb.2025.139191
收录类别	SCI
语种	英语
WOS研究方向	Chemistry ; Electrochemistry ; Instruments & Instrumentation
WOS类目	Chemistry, Analytical ; Electrochemistry ; Instruments & Instrumentation
WOS记录号	WOS:001629750600004
出版者	ELSEVIER SCIENCE SA
引用统计
文献类型	期刊论文
条目标识符	http://119.78.100.204/handle/2XEOYT63/42970
专题	中国科学院计算技术研究所
通讯作者	Zhao, Yang; Zhao, Haiping
作者单位	1.Chinese Acad Sci, Inst Microelect, Beijing 100029, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Microsyst & Informat Technol, 2020 X Lab, Shanghai 200050, Peoples R China 3.Capital Med Univ, Beijing Luhe Hosp, Dept Neurol, Beijing 101149, Peoples R China 4.Capital Med Univ, Xuanwu Hosp, Inst Cerebrovascular Dis Res, Beijing 100053, Peoples R China 5.Chinese Acad Sci, Inst Comp Technol, State Key Lab Comp Architecture, Beijing 101125, Peoples R China
推荐引用方式 GB/T 7714	Ye, Yifei,Geng, Xiaokun,Kong, Qi,et al. Label-free detection of macrophage polarization via specific membrane capacitance using high-throughput microfluidic impedance cytometry[J]. SENSORS AND ACTUATORS B-CHEMICAL,2026,449:11.
APA	Ye, Yifei.,Geng, Xiaokun.,Kong, Qi.,Li, Xue.,Zhi, Tian.,...&Zhao, Haiping.(2026).Label-free detection of macrophage polarization via specific membrane capacitance using high-throughput microfluidic impedance cytometry.SENSORS AND ACTUATORS B-CHEMICAL,449,11.
MLA	Ye, Yifei,et al."Label-free detection of macrophage polarization via specific membrane capacitance using high-throughput microfluidic impedance cytometry".SENSORS AND ACTUATORS B-CHEMICAL 449(2026):11.