Biochar amendment enhances water use efficiency in alfalfa<i> (Medicago</i><i> sativa</i> L.) under partial root-zone drying irrigation by modulating abscisic acid signaling and photosynthetic performance

doi:10.1016/j.envexpbot.2025.106244

	Biochar amendment enhances water use efficiency in alfalfa (Medicago sativa L.) under partial root-zone drying irrigation by modulating abscisic acid signaling and photosynthetic performance
	Zhong, Shangzhi 1,2,3; Zhang, Xiang 4; Hou, Pengxin 1; Ouyang, Jianghan 1; Rakotoson, Tovohery 5; Zheng, Congcong 6,7; Tao, Qibo 1,2,3; Sun, Juan 1,2,3
	2025-10-01
发表期刊	ENVIRONMENTAL AND EXPERIMENTAL BOTANY
ISSN	0098-8472
卷号	238 页码:15
摘要	Deficit irrigation can potentially increase plant water use efficiency by regulating stomatal morphology and photosynthetic physiology, whereas the combined effects of biochar amendment and deficit irrigation on alfalfa growth and leaf physiology remain largely unknown in salt-affected soil. A split-root pot experiment was implemented to investigate the effect of biochar amendments (WSB: wheat straw biochar; CSB: corn straw biochar) and irrigation regimes (FI: full irrigation; DI: deficit irrigation, 70 % of FI on the entire root zone; PRDI: partial root-zone drying irrigation, only irrigating half of the root zone with soil water content maintained at the same level as that under DI) on the leaf morpho-physiological traits and water use efficiency of alfalfa (Medicago sativa L.). DI and PRDI exhibited a similar trend, with both leading to a significant reduction in stomatal conductance (gs), carbon isotope discrimination (Delta 13Cleaf), and net CO2 assimilation rate (A) by altering stomatal traits and elevating leaf abscisic acid concentration ([ABA]leaf), resulting in lower biomass accumulation. In contrast, biochar amendment of WSB and CSB significantly improved soil water-holding capacity, root water uptake and leaf water status, resulting in lower [ABA]leaf and enhanced stomatal density (SD), stomatal size (SS) and Delta 13Cleaf. Notably, PRDI combined with biochar amendment substantially enhanced leaf intrinsic WUE (A/gs) and long-term WUE indicated by lower Delta 13Cleaf, thereby increasing plant-scale WUE (WUEplant) by 39-56 % compared to non-biochar-amended under PRDI treatment. Overall, co-application of biochar amendment and deficit irrigation facilitates more efficient and ecologically sustainable alfalfa management in salt-affected soil. Future studies should investigate long-term effects, underlying mechanisms, and large-scale applicability across diverse environmental contexts.
关键词	Alternate partial root-zone drying irrigation Abscisic acid Stomatal conductance Stomatal morphology Carbon isotope discrimination
DOI	10.1016/j.envexpbot.2025.106244
收录类别	SCI
语种	英语
资助项目	Qingdao Agricultural University high-level talent research fund[CARS-34] ; Joachim Herz Foundation[1121026] ; European Research Council (ERC) under the European Union[850035] ; [101003125]
WOS研究方向	Plant Sciences ; Environmental Sciences & Ecology
WOS类目	Plant Sciences ; Environmental Sciences
WOS记录号	WOS:001584863400001
出版者	PERGAMON-ELSEVIER SCIENCE LTD
引用统计
文献类型	期刊论文
条目标识符	http://119.78.100.204/handle/2XEOYT63/41653
专题	中国科学院计算技术研究所期刊论文_英文
通讯作者	Sun, Juan
作者单位	1.Qingdao Agr Univ, Coll Grassland Sci, Qingdao 266109, Shandong, Peoples R China 2.Qingdao Agr Univ, Shandong Key Lab Germplasm Innovat Saline Alkaline, Qingdao 266109, Shandong, Peoples R China 3.Qingdao Agr Univ, Qingdao Key Lab Specialty Plant Germplasm Innovat, Qingdao 266109, Shandong, Peoples R China 4.Jiangxi Univ Chinese Med, Coll Life Sci, Sch Tradit Chinese Med, Nanchang 330004, Jiangxi, Peoples R China 5.Int Rice Res Inst IRRI, Vietnam Off, Agr Genet Inst, KM 2 Duong Pham Van Dong, Hanoi 143311, Vietnam 6.Chinese Acad Sci, Inst Comp Technol, Beijing 100190, Peoples R China 7.INRAE, UMR, ISPA, F-33140 Villenave Dornon, France
推荐引用方式 GB/T 7714	Zhong, Shangzhi,Zhang, Xiang,Hou, Pengxin,et al. Biochar amendment enhances water use efficiency in alfalfa (Medicago sativa L.) under partial root-zone drying irrigation by modulating abscisic acid signaling and photosynthetic performance[J]. ENVIRONMENTAL AND EXPERIMENTAL BOTANY,2025,238:15.
APA	Zhong, Shangzhi.,Zhang, Xiang.,Hou, Pengxin.,Ouyang, Jianghan.,Rakotoson, Tovohery.,...&Sun, Juan.(2025).Biochar amendment enhances water use efficiency in alfalfa (Medicago sativa L.) under partial root-zone drying irrigation by modulating abscisic acid signaling and photosynthetic performance.ENVIRONMENTAL AND EXPERIMENTAL BOTANY,238,15.
MLA	Zhong, Shangzhi,et al."Biochar amendment enhances water use efficiency in alfalfa (Medicago sativa L.) under partial root-zone drying irrigation by modulating abscisic acid signaling and photosynthetic performance".ENVIRONMENTAL AND EXPERIMENTAL BOTANY 238(2025):15.