Cost-Effective DNA Storage System with DNA Movable Type

doi:10.1002/advs.202411354

	Cost-Effective DNA Storage System with DNA Movable Type
	Wang, Chenyang 1,2,3; Wei, Di 1,2; Wei, Zheng 4; Yang, Dongxin 5; Xing, Jing 4; Wang, Yunze 1,2,3; Wang, Xiaotong 1,2; Wang, Pei 5; Ma, Guannan 1,2; Zhang, Xinru 3,4; Li, Haolan 5; Tang, Chuan 5; Hou, Pengfei 5; Wang, Jie 1,2,3; Gao, Renjun 6; Xie, Guiqiu 6; Li, Cuidan 1,2; Ju, Yingjiao 1,2,3; Wang, Peihan 1,2,3; Yue, Liya 1,2; Zhao, Yongliang 1,2; Sheng, Yongjie 6; Xiao, Jingfa 2,3,7; Niu, Haitao 8; Xu, Sihong 9; Yang, Huaiyi 3,10; Liu, Di 3,11; Duan, Bo 3,4,5; Bu, Dongbo 3,4,5,12; Tan, Guangming 3,4,5; Chen, Fei 1,2,3,8,13
	2024-11-18
发表期刊	ADVANCED SCIENCE
页码	15
摘要	In the face of exponential data growth, DNA-based storage offers a promising solution for preserving big data. However, most existing DNA storage methods, akin to traditional block printing, require costly chemical synthesis for each individual data file, adopting a sequential, one-time-use synthesis approach. To overcome these limitations, a novel, cost-effective "DNA-movable-type storage" system, inspired by movable type printing, is introduced. This system utilizes prefabricated DNA movable types-short, double-stranded DNA oligonucleotides encoding specific payload, address, and checksum data. These DNA-MTs are enzymatically ligated/assembled into cohesive sequences, termed "DNA movable type blocks," streamlining the assembly process with the automated BISHENG-1 DNA-MT inkjet printer. Using BISHENG-1, 43.7 KB of data files are successfully printed, assembled, stored, and accurately retrieved in diverse formats (text, image, audio, and video) in vitro and in vivo, using only 350 DNA-MTs. Notably, each DNA-MT, synthesized once (2 OD), can be used up to 10000 times, reducing costs to $122/MB-outperforming existing DNA storage methods. This innovation circumvents the need to synthesize entire DNA sequences encoding files from scratch, offering significant cost and efficiency advantages. Furthermore, it has considerable untapped potential to advance a robust DNA storage system, better meeting the extensive data storage demands of the big-data era.
关键词	DNA data storage DNA movable type DNA-based storage
DOI	10.1002/advs.202411354
收录类别	SCI
语种	英语
资助项目	National Key Research and Development Program of China ; National Natural Science Foundation of China[T2125013] ; National Natural Science Foundation of China[31971389] ; National Natural Science Foundation of China[32271297] ; ICT Computer-X center ; [2020YFA0907000]
WOS研究方向	Chemistry ; Science & Technology - Other Topics ; Materials Science
WOS类目	Chemistry, Multidisciplinary ; Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary
WOS记录号	WOS:001356845300001
出版者	WILEY
引用统计
文献类型	期刊论文
条目标识符	http://119.78.100.204/handle/2XEOYT63/41187
专题	中国科学院计算技术研究所期刊论文_英文
通讯作者	Duan, Bo; Bu, Dongbo; Tan, Guangming; Chen, Fei
作者单位	1.China Natl Ctr Bioinformat, Beijing, Peoples R China 2.Chinese Acad Sci, Beijing Inst Genom, Beijing 100101, Peoples R China 3.Univ Chinese Acad Sci, Beijing 101408, Peoples R China 4.Chinese Acad Sci, SKLP, Inst Comp Technol, Beijing, Peoples R China 5.Western Inst Comp Technol, Chongqing 401121, Peoples R China 6.Jilin Univ, Sch Life Sci, Key Lab Mol Enzymol & Engn, Minist Educ, Changchun 130012, Jilin, Peoples R China 7.China Natl Ctr Bioinformat, Natl Genom Data Ctr, Beijing 100101, Peoples R China 8.Jinan Univ, Key Lab Viral Pathogenesis & Infect Prevent & Cont, Minist Educ, Guangzhou 510632, Peoples R China 9.Natl Inst Food & Drug Control, Inst Vitro Diagnost Control, Div Vitro Diagnost Infect Dis 2, Beijing 100050, Peoples R China 10.Chinese Acad Sci, Inst Microbiol, State Key Lab Mycol, Dept Microbial Physiol & Metab Engn, Beijing 100101, Peoples R China 11.Chinese Acad Sci, Wuhan Inst Virol, Ctr Biosafety Mega Sci, CAS Key Lab Special Pathogens & Biosafety, Wuhan 430071, Peoples R China 12.Cent China Inst Artificial Intelligence, Zhengzhou 450046, Peoples R China 13.Xinjiang Med Univ, Affiliated Hosp 1, Clin Med Inst, State Key Lab Pathogenesis Prevent & Treatment Hig, Urumqi 830054, Xinjiang, Peoples R China
推荐引用方式 GB/T 7714	Wang, Chenyang,Wei, Di,Wei, Zheng,et al. Cost-Effective DNA Storage System with DNA Movable Type[J]. ADVANCED SCIENCE,2024:15.
APA	Wang, Chenyang.,Wei, Di.,Wei, Zheng.,Yang, Dongxin.,Xing, Jing.,...&Chen, Fei.(2024).Cost-Effective DNA Storage System with DNA Movable Type.ADVANCED SCIENCE,15.
MLA	Wang, Chenyang,et al."Cost-Effective DNA Storage System with DNA Movable Type".ADVANCED SCIENCE (2024):15.