Institute of Computing Technology, Chinese Academy IR
| Auto-focus scanning surface plasmon resonance microscopy | |
| Sun, Sheng1,2; Liu, Pengbin1,2; Hu, Jingfang3; Zhang, Lingqian1; Li, Mingxiao1; Lu, Xinchao1,4; Zhao, Yang1,4; Zhi, Tian5; Huang, Chengjun1,2 | |
| 2025-04-07 | |
| 发表期刊 | OPTICS EXPRESS
 |
| ISSN | 1094-4087 |
| 卷号 | 33期号:7页码:16551-16561 |
| 摘要 | Wide-field inspection, nano detection, and real-time observation are essential for is a label-free, real-time, and nano-imaging method that is widely employed for the dynamic detection of nanoscale biomolecules. The field of view (FOV) of SPRM is limited by the usage of high NA objectives, and a scanning SPRM is required to obtain a large FOV. However, during the scanning, the focus drift introduced by the mechanical vibrations blurs the imaging quality of SPRM, making the detection deviate from the true status. To this end, this paper presents the development of autofocus scanning SPRM (AFS-SPRM) that is capable of performing automated real-time focus drift correction during auto-scanning, thereby enabling high-quality SPRM imaging with large FOV. Only 80 ms is taken to process each defocusing event, and the ability to maintain focus has been improved by 30 times by comparison with SPRM. The AFS-SPRM was successfully employed to distinguish nanoparticles of different sizes and to observe the changes of macrophages in a culture medium containing nanoparticles. This investigation illustrates the superior imaging capabilities of AFS-SPRM and demonstrates its potential for observing interactions between biomolecules at the nanoscale. |
| DOI | 10.1364/OE.557410 |
| 收录类别 | SCI |
| 语种 | 英语 |
| 资助项目 | National Natural Science Foundation of China-Yunnan Joint Fund[62171441] ; National Natural Science Foundation of China-Yunnan Joint Fund[62474193] ; National Natural Science Foundation of China-Yunnan Joint Fund[62275264] ; National Natural Science Foundation of China-Yunnan Joint Fund[U20A20227] ; Opening Project of Key Laboratory of Fabrication Technologies for Integrated Circuits ; Chinese Academy of Sciences ; CAS-CSIRO Partnership Joint Project[172511KYSB20210008] |
| WOS研究方向 | Optics |
| WOS类目 | Optics |
| WOS记录号 | WOS:001476276900010 |
| 出版者 | Optica Publishing Group |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://119.78.100.204/handle/2XEOYT63/40613 |
| 专题 | 中国科学院计算技术研究所期刊论文_英文 |
| 通讯作者 | Lu, Xinchao |
| 作者单位 | 1.Chinese Acad Sci, Inst Microelect, Beijing 100029, Peoples R China 2.Univ Chinese Acad Sci, Beijing 101408, Peoples R China 3.Beijing Informat Sci & Technol Univ, Beijing Key Lab Sensor, Beijing 100101, Peoples R China 4.Chinese Acad Sci, Key Lab Fabricat Technol Integrated Circuits, Beijing, Peoples R China 5.Chinese Acad Sci, Inst Comp Technol, State Key Lab Comp Architecture, Beijing 101125, Peoples R China |
| 推荐引用方式 GB/T 7714 | Sun, Sheng,Liu, Pengbin,Hu, Jingfang,et al. Auto-focus scanning surface plasmon resonance microscopy[J]. OPTICS EXPRESS,2025,33(7):16551-16561. |
| APA | Sun, Sheng.,Liu, Pengbin.,Hu, Jingfang.,Zhang, Lingqian.,Li, Mingxiao.,...&Huang, Chengjun.(2025).Auto-focus scanning surface plasmon resonance microscopy.OPTICS EXPRESS,33(7),16551-16561. |
| MLA | Sun, Sheng,et al."Auto-focus scanning surface plasmon resonance microscopy".OPTICS EXPRESS 33.7(2025):16551-16561. |
| 条目包含的文件 | 条目无相关文件。 | |||||
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