Top-down Proteomics: The Large-scale Proteoform Identification

	Top-down Proteomics: The Large-scale Proteoform Identification
	Sun RX(孙瑞祥); Luo L(罗兰); Chi H(迟浩); Liu C(刘超); He SM(贺思敏)
	2015
发表期刊	PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS
ISSN	1000-3282
卷号	42 期号:2 页码:101
摘要	With the rapid advancement of the high resolution mass spectrometry, top-down proteomics becomes the reality. Proteome research on the intact protein level will provide more precise and more abundant biological information. For example, it can detect the relationship between the multiple post-translational modifications. Due to the genetic mutation, alternative splicing of RNA and various post-translational modifications, one gene may produce multiple protein forms, now called 'proteoforms'. Top-down proteomics will help identify the proteoforms. The three pillar technologies in top-down proteomics are separation, mass spectrometry and bioinformatics from the point of view on the entire proteins. This paper reviews these technologies and puts more emphases on the bioinformatics related topics, including the mass spectral preprocessing, the database searching algorithms and the localization of post-translational modifications.
关键词	DISSOCIATION MASS-SPECTROMETRY ELECTRON-CAPTURE DISSOCIATION CHARGED PROTEIN CATIONS INTACT PROTEINS BOTTOM-UP PEPTIDE IDENTIFICATION MONOCLONAL-ANTIBODIES STRUCTURAL-ANALYSIS MEMBRANE-PROTEINS YEAST PROTEOME top-down proteomics tandem mass spectrometry bioinformatics protein identification
语种	英语
资助项目	[National Basic Research Program of China]
文献类型	期刊论文
条目标识符	http://119.78.100.204/handle/2XEOYT63/32040
专题	中国科学院计算技术研究所期刊论文_中文
作者单位	中国科学院计算技术研究所
第一作者单位	中国科学院计算技术研究所
推荐引用方式 GB/T 7714	Sun RX,Luo L,Chi H,et al. Top-down Proteomics: The Large-scale Proteoform Identification[J]. PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,2015,42(2):101.
APA	孙瑞祥,罗兰,迟浩,刘超,&贺思敏.(2015).Top-down Proteomics: The Large-scale Proteoform Identification.PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,42(2),101.
MLA	孙瑞祥,et al."Top-down Proteomics: The Large-scale Proteoform Identification".PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS 42.2(2015):101.