ECD/ETD-based Tandem Mass Spectrometry in Proteomics

doi:10.3724/SP.J.1206.2009.00352

	ECD/ETD-based Tandem Mass Spectrometry in Proteomics
	Sun Rui-Xiang 1; Dong Meng-Qiu 2; Chi Hao 1; Yang Bing 2; Xiu Li-Yun 1; Wang Le-Heng 1; Fu Yan 1; He Si-Min 1
	2010
发表期刊	PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS
ISSN	1000-3282
卷号	37 期号:1 页码:94-102
摘要	Proteomics has propelled the rapid development of mass spectrometry (MS) in the past ten years. Conversely, the advancement of MS has greatly expanded the horizon of proteomics research by allowing it to address questions never touched before. Standing out among the recent technological innovations in MS are electron capture dissociation (ECD) and electron transfer dissociation (ETD). These electron-based fragmentation methods have shown a great potential for analysis of proteolytic peptides as well as intact proteins. A promising future lies ahead for ECD- or ETD-based techniques in proteomics research, especially in studies of protein post-translational modifications and top-down analysis of intact proteins. The mechanism, instrumentation and application of ECD/ETD, along with the spectral characteristics and data analysis of peptides ECD/ETD spectra were reviewed. The current issues in ECD/ETD applications or method development, the challenges for software development, and the outlook of these techniques in future research were also discussed.
关键词	electron capture dissociation electron transfer dissociation collision induced dissociation tandem mass spectrometry computational proteomics
DOI	10.3724/SP.J.1206.2009.00352
收录类别	SCI
语种	英语
资助项目	Hi-Tech Research and Development Program of China[2008AA02Z309] ; Hi-Tech Research and Development Program of China[2007AA02Z315] ; Hi-Tech Research and Development Program of China[2007AA02Z1A7] ; National Basic Research Program of China[2010CB912701] ; The Chinese Academy of Sciences Knowledge Innovative Project[KGGX1-YW-13]
WOS研究方向	Biochemistry & Molecular Biology ; Biophysics
WOS类目	Biochemistry & Molecular Biology ; Biophysics
WOS记录号	WOS:000273981400014
出版者	CHINESE ACAD SCIENCES, INST BIOPHYSICS
引用统计	被引频次：11[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://119.78.100.204/handle/2XEOYT63/12313
专题	中国科学院计算技术研究所期刊论文_英文
通讯作者	Sun Rui-Xiang
作者单位	1.Chinese Acad Sci, Inst Comp Technol, Key Lab Intelligent Informat Proc, Beijing 100190, Peoples R China 2.Natl Inst Biol Sci, Beijing 102206, Peoples R China
推荐引用方式 GB/T 7714	Sun Rui-Xiang,Dong Meng-Qiu,Chi Hao,et al. ECD/ETD-based Tandem Mass Spectrometry in Proteomics[J]. PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,2010,37(1):94-102.
APA	Sun Rui-Xiang.,Dong Meng-Qiu.,Chi Hao.,Yang Bing.,Xiu Li-Yun.,...&He Si-Min.(2010).ECD/ETD-based Tandem Mass Spectrometry in Proteomics.PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,37(1),94-102.
MLA	Sun Rui-Xiang,et al."ECD/ETD-based Tandem Mass Spectrometry in Proteomics".PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS 37.1(2010):94-102.