Alu-directed transcriptional regulation of some novel miRNAs

doi:10.1186/1471-2164-10-563

	Alu-directed transcriptional regulation of some novel miRNAs
	Gu, Tong J.1,3; Yi, Xiang 1,3; Zhao, Xi W.1,3; Zhao, Yi 2; Yin, James Q.1
	2009-11-30
发表期刊	BMC GENOMICS
ISSN	1471-2164
卷号	10 页码:13
摘要	Background: Despite many studies on the biogenesis, molecular structure and biological functions of microRNAs, little is known about the transcriptional regulatory mechanisms controlling the spatiotemporal expression pattern of human miRNA gene loci. Several lines of experimental results have indicated that both polymerase II (Pol-II) and polymerase III (Pol-III) may be involved in transcribing miRNAs. Here, we assessed the genomic evidence for Alu-directed transcriptional regulation of some novel miRNA genes in humans. Our data demonstrate that the expression of these Alu-related miRNAs may be modulated by Pol-III. Results: We present a comprehensive exploration of the Alu-directed transcriptional regulation of some new miRNAs. Using a new computational approach, a variety of Alu-related sequences from multiple sources were pooled and filtered to obtain a subset containing Alu elements and characterized miRNA genes for which there is clear evidence of full-length transcription (embedded in EST). We systematically demonstrated that 73 miRNAs including five known ones may be transcribed by Pol-III through Alu or MIR. Among the new miRNAs, 33 were determined by high-throughput Solexa sequencing. Real-time TaqMan PCR and Northern blotting verified that three newly identified miRNAs could be induced to co-express with their upstream Alu transcripts by heat shock or cycloheximide. Conclusion: Through genomic analysis, Solexa sequencing and experimental validation, we have identified candidate sequences for Alu-related miRNAs, and have found that the transcription of these miRNAs could be governed by Pol-III. Thus, this study may elucidate the mechanisms by which the expression of a class of small RNAs may be regulated by their upstream repeat elements.
DOI	10.1186/1471-2164-10-563
收录类别	SCI
语种	英语
资助项目	Chinese Academy of Sciences[2006AA02Z131] ; Chinese Academy of Sciences[2007AA02Z181] ; National Natural Science Foundation of China[30671042] ; National Natural Science Foundation of China[30871250]
WOS研究方向	Biotechnology & Applied Microbiology ; Genetics & Heredity
WOS类目	Biotechnology & Applied Microbiology ; Genetics & Heredity
WOS记录号	WOS:000272983200001
出版者	BIOMED CENTRAL LTD
引用统计	被引频次：42[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://119.78.100.204/handle/2XEOYT63/11887
专题	中国科学院计算技术研究所期刊论文_英文
通讯作者	Yin, James Q.
作者单位	1.Chinese Acad Sci, Inst Biophys, Ctr Comp & Syst Biol, Natl Lab Biomacromol, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Inst Comp Technol, Bioinformat Res Grp, Beijing 100190, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing 100080, Peoples R China
推荐引用方式 GB/T 7714	Gu, Tong J.,Yi, Xiang,Zhao, Xi W.,et al. Alu-directed transcriptional regulation of some novel miRNAs[J]. BMC GENOMICS,2009,10:13.
APA	Gu, Tong J.,Yi, Xiang,Zhao, Xi W.,Zhao, Yi,&Yin, James Q..(2009).Alu-directed transcriptional regulation of some novel miRNAs.BMC GENOMICS,10,13.
MLA	Gu, Tong J.,et al."Alu-directed transcriptional regulation of some novel miRNAs".BMC GENOMICS 10(2009):13.