Preliminary expression profile of cytokines in brain tissue of BALB/c mice with Angiostrongylus cantonensis infection

doi:10.1186/s13071-015-0939-6

	Preliminary expression profile of cytokines in brain tissue of BALB/c mice with Angiostrongylus cantonensis infection
	Yu, Liping 1,2; Wu, Xiaoying 3; Wei, Jie 2,3; Liao, Qi 4; Xu, Lian 2,3; Luo, Siqi 2,3; Zeng, Xin 2,3; Zhao, Yi 5; Lv, Zhiyue 2,3; Wu, Zhongdao 2,3
	2015-06-14
发表期刊	PARASITES & VECTORS
ISSN	1756-3305
卷号	8 页码:10
摘要	Background: Angiostrongylus cantonensis (A. cantonensis) infection can result in increased risk of eosinophilic meningitis. Accumulation of eosinophils and inflammation can result in the A. cantonensis infection playing an important role in brain tissue injury during this pathological process. However, underlying mechanisms regarding the transcriptomic responses during brain tissue injury caused by A. cantonensis infection are yet to be elucidated. This study is aimed at identifying some genomic and transcriptomic factors influencing the accumulation of eosinophils and inflammation in the mouse brain infected with A. cantonensis. Methods: An infected mouse model was prepared based on our laboratory experimental process, and then the mouse brain RNA Libraries were constructed for deep Sequencing with Illumina Genome Analyzer. The raw data was processed with a bioinformatics' pipeline including Refseq genes expression analysis using cufflinks, annotation and classification of RNAs, lncRNA prediction as well as analysis of co-expression network. The analysis of Refseq data provides the measure of the presence and prevalence of transcripts from known and previously unknown genes. Results: This study showed that Cys-Cys (CC) type chemokines such as CCL2, CCL8, CCL1, CCL24, CCL11, CCL7, CCL12 and CCL5 were elevated significantly at the late phase of infection. The up-regulation of CCL2 indicated that the worm of A. cantonensis had migrated into the mouse brain at an early infection phase. CCL2 could be induced in the brain injury during migration and CCL2 might play a major role in the neuropathic pain caused by A. cantonensis infection. The up-regulated expression of IL-4, IL-5, IL-10, and IL-13 showed Th2 cell predominance in immunopathological reactions at late infection phase in response to infection by A. cantonensis. These different cytokines can modulate and inhibit each other and function as a network with the specific potential to drive brain eosinophilic inflammation. The increase of ATF-3 expression at 21 dpi suggested the injury of neuronal cells at late phase of infection. 1217 new potential lncRNA were candidates of interest for further research. Conclusions: These cytokine networks play an important role in the development of central nervous system inflammation caused by A. cantonensis infection.
关键词	Angiostrongylus cantonensis Eosinophil Mouse model Cytokine Chemokine
DOI	10.1186/s13071-015-0939-6
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[81271855] ; National Natural Science Foundation of China[81261160324] ; National Natural Science Foundation of China[81371836] ; National Basic Research Program of China[2010CB530004]
WOS研究方向	Parasitology
WOS类目	Parasitology
WOS记录号	WOS:000356667400003
出版者	BIOMED CENTRAL LTD
引用统计	被引频次：24[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://119.78.100.204/handle/2XEOYT63/9635
专题	中国科学院计算技术研究所期刊论文_英文
通讯作者	Wu, Zhongdao
作者单位	1.Three Gorges Univ, Sch Med, Dept Prevent Med, Yichang, Peoples R China 2.Sun Yat Sen Univ, Zhongshan Sch Med, Dept Parasitol, Guangzhou 510275, Guangdong, Peoples R China 3.Sun Yat Sen Univ, Key Lab Trop Dis Control, Minist Educ, Guangzhou 510275, Guangdong, Peoples R China 4.Ningbo Univ, Sch Med, Dept Prevent Med, Ningbo 315211, Zhejiang, Peoples R China 5.Chinese Acad Sci, Inst Comp Technol, Adv Comp Res Lab, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Yu, Liping,Wu, Xiaoying,Wei, Jie,et al. Preliminary expression profile of cytokines in brain tissue of BALB/c mice with Angiostrongylus cantonensis infection[J]. PARASITES & VECTORS,2015,8:10.
APA	Yu, Liping.,Wu, Xiaoying.,Wei, Jie.,Liao, Qi.,Xu, Lian.,...&Wu, Zhongdao.(2015).Preliminary expression profile of cytokines in brain tissue of BALB/c mice with Angiostrongylus cantonensis infection.PARASITES & VECTORS,8,10.
MLA	Yu, Liping,et al."Preliminary expression profile of cytokines in brain tissue of BALB/c mice with Angiostrongylus cantonensis infection".PARASITES & VECTORS 8(2015):10.