Pulsed Azidohomoalanine Labeling in Mammals (PALM) Detects Changes in Liver-Specific LKB1 Knockout Mice

doi:10.1021/acs.jproteome.5b00653

	Pulsed Azidohomoalanine Labeling in Mammals (PALM) Detects Changes in Liver-Specific LKB1 Knockout Mice
	McClatchy, Daniel B.1; Ma, Yuanhui 1; Liu, Chao 2; Stein, Benjamin D.1; Martinez-Bartolome, Salvador 1; Vasquez, Debbie 3; Hellberg, Kristina 3; Shaw, Reuben J.3,4; Yates, John R., III 1
	2015-11-01
发表期刊	JOURNAL OF PROTEOME RESEARCH
ISSN	1535-3893
卷号	14 期号:11 页码:4815-4822
摘要	Quantification of proteomes by mass spectrometry has proven to be useful to study human pathology recapitulated in cellular or animal models of disease. Enriching and quantifying newly synthesized proteins (NSPs) at set time points by mass spectrometry has the potential to identify important early regulatory or expression changes associated with disease states or perturbations. NSP can be enriched from proteomes by employing pulsed introduction of the non-canonical amino acid, azidohomoalanine (AHA). We demonstrate that pulsed introduction of AHA in the feed of mice can label and identify NSP from multiple tissues. Furthermore, we quantitate differences in new protein expression resulting from CRE-LOX initiated knockout of LKB1 in mouse livers. Overall, the PALM strategy allows for the first time in vivo labeling of mouse tissues to differentiate protein synthesis rates at discrete time points.
关键词	azidohomoalanine newly synthesized proteins proteomics mass spectrometry BONCAT LKB1 quantitation
DOI	10.1021/acs.jproteome.5b00653
收录类别	SCI
语种	英语
资助项目	National Institutes of Health[P41 GM103533] ; National Institutes of Health[R01 MH067880] ; National Institutes of Health[1 R01 MH100175] ; National Institutes of Health[HHSN268201000035C] ; National Institutes of Health[R01DK080425] ; National Key Basic Research and Development Program of China (973)[2012CB910602] ; George E. Hewitt Foundation for Medical Research
WOS研究方向	Biochemistry & Molecular Biology
WOS类目	Biochemical Research Methods
WOS记录号	WOS:000364435100033
出版者	AMER CHEMICAL SOC
引用统计	被引频次：61[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://119.78.100.204/handle/2XEOYT63/9170
专题	中国科学院计算技术研究所期刊论文_英文
通讯作者	Yates, John R., III
作者单位	1.Scripps Res Inst, Dept Chem Physiol & Mol & Cellular Mol Biol, La Jolla, CA 92037 USA 2.Chinese Acad Sci, Inst Comp Technol, Key Lab Intelligent Informat Proc, Beijing 100190, Peoples R China 3.Salk Inst Biol Studies, Mol & Cell Biol Lab, La Jolla, CA 92037 USA 4.Salk Inst Biol Studies, Howard Hughes Med Inst, La Jolla, CA 92037 USA
推荐引用方式 GB/T 7714	McClatchy, Daniel B.,Ma, Yuanhui,Liu, Chao,et al. Pulsed Azidohomoalanine Labeling in Mammals (PALM) Detects Changes in Liver-Specific LKB1 Knockout Mice[J]. JOURNAL OF PROTEOME RESEARCH,2015,14(11):4815-4822.
APA	McClatchy, Daniel B..,Ma, Yuanhui.,Liu, Chao.,Stein, Benjamin D..,Martinez-Bartolome, Salvador.,...&Yates, John R., III.(2015).Pulsed Azidohomoalanine Labeling in Mammals (PALM) Detects Changes in Liver-Specific LKB1 Knockout Mice.JOURNAL OF PROTEOME RESEARCH,14(11),4815-4822.
MLA	McClatchy, Daniel B.,et al."Pulsed Azidohomoalanine Labeling in Mammals (PALM) Detects Changes in Liver-Specific LKB1 Knockout Mice".JOURNAL OF PROTEOME RESEARCH 14.11(2015):4815-4822.