pGlyco: a pipeline for the identification of intact N-glycopeptides by using HCD-and CID-MS/MS and MS3

doi:10.1038/srep25102

	pGlyco: a pipeline for the identification of intact N-glycopeptides by using HCD-and CID-MS/MS and MS3
	Zeng, Wen-Feng 1,2; Liu, Ming-Qi 3; Zhang, Yang 3; Wu, Jian-Qiang 1,2; Fang, Pan 3; Peng, Chao 4; Nie, Aiying 5; Yan, Guoquan 3; Cao, Weiqian 3; Liu, Chao 1; Chi, Hao 1; Sun, Rui-Xiang 1; Wong, Catherine C. L.4; He, Si-Min 1,2; Yang, Pengyuan 3
	2016-05-03
发表期刊	SCIENTIFIC REPORTS
ISSN	2045-2322
卷号	6 页码:10
摘要	Confident characterization of the microheterogeneity of protein glycosylation through identification of intact glycopeptides remains one of the toughest analytical challenges for glycoproteomics. Recently proposed mass spectrometry (MS)-based methods still have some defects such as lack of the false discovery rate (FDR) analysis for the glycan identification and lack of sufficient fragmentation information for the peptide identification. Here we proposed pGlyco, a novel pipeline for the identification of intact glycopeptides by using complementary MS techniques: 1) HCD-MS/MS followed by product-dependent CID-MS/MS was used to provide complementary fragments to identify the glycans, and a novel target-decoy method was developed to estimate the false discovery rate of the glycan identification; 2) data-dependent acquisition of MS3 for some most intense peaks of HCD-MS/MS was used to provide fragments to identify the peptide backbones. By integrating HCD-MS/MS, CID-MS/MS and MS3, intact glycopeptides could be confidently identified. With pGlyco, a standard glycoprotein mixture was analyzed in the Orbitrap Fusion, and 309 non-redundant intact glycopeptides were identified with detailed spectral information of both glycans and peptides.
DOI	10.1038/srep25102
收录类别	SCI
语种	英语
资助项目	National Basic Research Program of China[2011CB910600] ; National Basic Research Program of China[2013CB911203] ; National Basic Research Program of China[2010CB912701] ; National Basic Research Program of China[2011CB910604] ; National Natural Science Foundation of China[21227805] ; National Natural Science Foundation of China[31300680] ; Hi-Tech Research and Development Program of China[2014AA020902] ; Hi-Tech Research and Development Program of China[2012AA020203]
WOS研究方向	Science & Technology - Other Topics
WOS类目	Multidisciplinary Sciences
WOS记录号	WOS:000375290500001
出版者	NATURE PUBLISHING GROUP
引用统计	被引频次：79[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://119.78.100.204/handle/2XEOYT63/8538
专题	中国科学院计算技术研究所期刊论文_英文
通讯作者	Wong, Catherine C. L.; He, Si-Min; Yang, Pengyuan
作者单位	1.Chinese Acad Sci, Inst Comp Technol, Key Lab Intelligent Informat Proc, Beijing, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.Fudan Univ, Inst Biomed Sci, Shanghai 200433, Peoples R China 4.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Natl Ctr Prot Sci Shanghai, Shanghai, Peoples R China 5.Thermo Fisher Sci Co Ltd, Shanghai, Peoples R China
推荐引用方式 GB/T 7714	Zeng, Wen-Feng,Liu, Ming-Qi,Zhang, Yang,et al. pGlyco: a pipeline for the identification of intact N-glycopeptides by using HCD-and CID-MS/MS and MS3[J]. SCIENTIFIC REPORTS,2016,6:10.
APA	Zeng, Wen-Feng.,Liu, Ming-Qi.,Zhang, Yang.,Wu, Jian-Qiang.,Fang, Pan.,...&Yang, Pengyuan.(2016).pGlyco: a pipeline for the identification of intact N-glycopeptides by using HCD-and CID-MS/MS and MS3.SCIENTIFIC REPORTS,6,10.
MLA	Zeng, Wen-Feng,et al."pGlyco: a pipeline for the identification of intact N-glycopeptides by using HCD-and CID-MS/MS and MS3".SCIENTIFIC REPORTS 6(2016):10.