In-depth mapping of the mouse brain N-glycoproteome reveals widespread N-glycosylation of diverse brain proteins

doi:10.18632/oncotarget.9737

	In-depth mapping of the mouse brain N-glycoproteome reveals widespread N-glycosylation of diverse brain proteins
	Fang, Pan 1,2; Wang, Xin-jian 3,4; Xue, Yu 5; Liu, Ming-qi 1,2; Zeng, Wen-feng 6; Zhang, Yang 1,2; Zhang, Lei 1,2; Gao, Xing 1,2; Yan, Guo-quan 5; Yao, Jun 1,2; Shen, Hua-li 1,2,7,8; Yang, Peng-yuan 1,2,5,7,8
	2016-06-21
发表期刊	ONCOTARGET
ISSN	1949-2553
卷号	7 期号:25 页码:38796-38809
摘要	N-glycosylation is one of the most prominent and abundant posttranslational modifications of proteins. It is estimated that over 50% of mammalian proteins undergo glycosylation. However, the analysis of N-glycoproteins has been limited by the available analytical technology. In this study, we comprehensively mapped the N-glycosylation sites in the mouse brain proteome by combining complementary methods, which included seven protease treatments, four enrichment techniques and two fractionation strategies. Altogether, 13492 N-glycopeptides containing 8386 N-glycosylation sites on 3982 proteins were identified. After evaluating the performance of the above methods, we proposed a simple and efficient workflow for large-scale N-glycosylation site mapping. The optimized workflow yielded 80% of the initially identified N-glycosylation sites with considerably less effort. Analysis of the identified N-glycoproteins revealed that many of the mouse brain proteins are N-glycosylated, including those proteins in critical pathways for nervous system development and neurological disease. Additionally, several important biomarkers of various diseases were found to be N-glycosylated. These data confirm that N-glycosylation is important in both physiological and pathological processes in the brain, and provide useful details about numerous N-glycosylation sites in brain proteins.
关键词	N-glycoproteomics mouse brain mass spectrometry brain physiological activities disease biomarker
DOI	10.18632/oncotarget.9737
收录类别	SCI
语种	英语
资助项目	S973 Program (973 Program)[2013CB910503] ; S973 Program (973 Program)[2013CB910802] ; MOST 863 Program[2014AA010902]
WOS研究方向	Oncology ; Cell Biology
WOS类目	Oncology ; Cell Biology
WOS记录号	WOS:000378229100110
出版者	IMPACT JOURNALS LLC
引用统计	被引频次：21[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://119.78.100.204/handle/2XEOYT63/8352
专题	中国科学院计算技术研究所期刊论文_英文
通讯作者	Shen, Hua-li; Yang, Peng-yuan
作者单位	1.Fudan Univ, Minhang Hosp, Shanghai 200433, Peoples R China 2.Fudan Univ, Inst Biomed Sci, Shanghai 200433, Peoples R China 3.Fudan Univ, State Key Lab Med Neurobiol, Inst Brain Sci, Shanghai 200433, Peoples R China 4.Fudan Univ, Collaborat Innovat Ctr Brain Sci, Shanghai 200433, Peoples R China 5.Fudan Univ, Dept Chem, Shanghai 200433, Peoples R China 6.Chinese Acad Sci, Inst Comp Technol, Key Lab Intelligent Informat Proc, Beijing, Peoples R China 7.Fudan Univ, Dept Syst Biol Med, Shanghai 200433, Peoples R China 8.Fudan Univ, Sch Basic Med Sci, Shanghai 200433, Peoples R China
推荐引用方式 GB/T 7714	Fang, Pan,Wang, Xin-jian,Xue, Yu,et al. In-depth mapping of the mouse brain N-glycoproteome reveals widespread N-glycosylation of diverse brain proteins[J]. ONCOTARGET,2016,7(25):38796-38809.
APA	Fang, Pan.,Wang, Xin-jian.,Xue, Yu.,Liu, Ming-qi.,Zeng, Wen-feng.,...&Yang, Peng-yuan.(2016).In-depth mapping of the mouse brain N-glycoproteome reveals widespread N-glycosylation of diverse brain proteins.ONCOTARGET,7(25),38796-38809.
MLA	Fang, Pan,et al."In-depth mapping of the mouse brain N-glycoproteome reveals widespread N-glycosylation of diverse brain proteins".ONCOTARGET 7.25(2016):38796-38809.