Comprehensive Transcriptome Analyses of the Fructose-Fed Syrian Golden Hamster Liver Provides Novel Insights into Lipid Metabolism

doi:10.1371/journal.pone.0162402

	Comprehensive Transcriptome Analyses of the Fructose-Fed Syrian Golden Hamster Liver Provides Novel Insights into Lipid Metabolism
	Li, Ziyang 1; Xiong, Chaoliang 1; Mo, Suo 1; Tian, Haiying 1; Yu, Mengqian 1; Mao, Tingting 1; Chen, Qian 1; Luo, Haitao 3; Li, Quanzhen 4,5; Lu, Jianxin 1,2; Zhao, Yi 1,3; Li, Wei 1,2
	2016-09-02
发表期刊	PLOS ONE
ISSN	1932-6203
卷号	11 期号:9 页码:19
摘要	Dyslipidemia has been widely proven to contribute to cardiovascular diseases and other metabolic disorders, especially in insulin resistance and type 2 diabetes. The overproduction of VLDL is a significant characteristic of dyslipidemia, indicating the dysfunction of hepatic lipid metabolism, from triglyceride synthesis to transport. The fructose-fed Syrian golden hamster is an established animal model for the study of VLDL assembly with insulin resistance, however, it remains unknown how VLDL production is regulated at the transcriptional level due to the absence of a complete hamster genome. Here, we performed deep sequencing and constructed an mRNA-miRNA-lncRNA interaction network of Syrian golden hamster liver in order to reveal the global transcription profile and find potential RNA molecular regulation of VLDL production. We identified 4,450 novel multi-exon hamster lncRNAs and 755 miRNAs expressed in liver. Additionally, 146 differentially expressed coding genes, 27 differentially expressed lncRNA genes, as well as 16 differentially expressed miRNAs were identified. We then constructed an mRNA-miRNA-lncRNA interaction network that may potentially regulate VLDL production, and interestingly found several micro-RNA-centered regulatory networks. In order to verify our interpretation, miR-486 was selected for further experiments. Overexpression or down-regulation of miR-486 in fructose-fed hamsters resulted in altered hepatic expression of proteins involved in VLDL production, and in modulated levels of circulating VLDL. Our findings implicated that miR-486 is a potential regulator of circulating VLDL levels. These results provide new insights and a valuable resource for further study of the molecular mechanisms of VLDL secretion.
DOI	10.1371/journal.pone.0162402
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[81170749] ; National Natural Science Foundation of China[31370795] ; Zhejiang Provincial Program for the Cultivation of High-level Innovative Health Talents ; Zhejiang Provincial Top Key Discipline in Clinical Medicine (Laboratory Medicine)
WOS研究方向	Science & Technology - Other Topics
WOS类目	Multidisciplinary Sciences
WOS记录号	WOS:000382877800056
出版者	PUBLIC LIBRARY SCIENCE
引用统计	被引频次：9[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://119.78.100.204/handle/2XEOYT63/8172
专题	中国科学院计算技术研究所期刊论文_英文
通讯作者	Lu, Jianxin; Zhao, Yi; Li, Wei
作者单位	1.Wenzhou Med Univ, Sch Lab Med & Life Sci, Key Lab Lab Med, Minist Educ China, Wenzhou 325035, Peoples R China 2.Wenzhou Med Univ, Zhejiang Prov Key Lab Med Genet, Wenzhou 325035, Peoples R China 3.Chinese Acad Sci, Adv Comp Res Ctr, Inst Comp Technol, Key Lab Intelligent Informat Proc,State Key Lab C, Beijing 100190, Peoples R China 4.Univ Texas Southwestern Med Ctr Dallas, Dept Immunol, Dallas, TX 75390 USA 5.Univ Texas Southwestern Med Ctr Dallas, Microarray Core Facil, Dallas, TX 75390 USA
推荐引用方式 GB/T 7714	Li, Ziyang,Xiong, Chaoliang,Mo, Suo,et al. Comprehensive Transcriptome Analyses of the Fructose-Fed Syrian Golden Hamster Liver Provides Novel Insights into Lipid Metabolism[J]. PLOS ONE,2016,11(9):19.
APA	Li, Ziyang.,Xiong, Chaoliang.,Mo, Suo.,Tian, Haiying.,Yu, Mengqian.,...&Li, Wei.(2016).Comprehensive Transcriptome Analyses of the Fructose-Fed Syrian Golden Hamster Liver Provides Novel Insights into Lipid Metabolism.PLOS ONE,11(9),19.
MLA	Li, Ziyang,et al."Comprehensive Transcriptome Analyses of the Fructose-Fed Syrian Golden Hamster Liver Provides Novel Insights into Lipid Metabolism".PLOS ONE 11.9(2016):19.