Phenotype-Genotype Association Analysis of ACTH-Secreting Pituitary Adenoma and Its Molecular Link to Patient Osteoporosis

doi:10.3390/ijms17101654

	Phenotype-Genotype Association Analysis of ACTH-Secreting Pituitary Adenoma and Its Molecular Link to Patient Osteoporosis
	Wang, Renzhi 1,2,3,4; Yang, Yakun 1,2; Sheng, Miaomiao 5,6; Bu, Dechao 7; Huang, Fengming 5,6; Liu, Xiaohai 1,2; Zhou, Cuiqi 4,8; Dai, Congxin 1,2,3; Sun, Bowen 1,2; Zhu, Jindong 5,6; Qiao, Yi 1; Yao, Yong 1; Zhu, Huijuan 9; Lu, Lin 9; Pan, Hui 9; Feng, Ming 1; Deng, Kan 1; Xing, Bing 1; Lian, Wei 1; Zhao, Yi 7; Jiang, Chengyu 2,5,6
	2016-10-01
发表期刊	INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
ISSN	1422-0067
卷号	17 期号:10 页码:16
摘要	Adrenocorticotrophin (ACTH)-secreting pituitary adenoma, also known as Cushing disease (CD), is rare and causes metabolic syndrome, cardiovascular disease and osteoporosis due to hypercortisolism. However, the molecular pathogenesis of CD is still unclear because of a lack of human cell lines and animal models. Here, we study 106 clinical characteristics and gene expression changes from 118 patients, the largest cohort of CD in a single-center. RNA deep sequencing is used to examine genotypic changes in nine paired female ACTH-secreting pituitary adenomas and adjacent nontumorous pituitary tissues (ANPT). We develop a novel analysis linking disease clinical characteristics and whole transcriptomic changes, using Pearson Correlation Coefficient to discover a molecular network mechanism. We report that osteoporosis is distinguished from the phenotype and genotype analysis. A cluster of genes involved in osteoporosis is identified using Pearson correlation coefficient analysis. Most of the genes are reported in the bone related literature, confirming the feasibility of phenotype-genotype association analysis, which could be used in the analysis of almost all diseases. Secreted phosphoprotein 1 (SPP1), collagen type I alpha 1 chain (COL1A1), 5'-nucleotidase ecto (NT5E), HtrA serine peptidase 1 (HTRA1) and angiopoietin 1 (ANGPT1) and their signalling pathways are shown to be involved in osteoporosis in CD patients. Our discoveries provide a molecular link for osteoporosis in CD patients, and may open new potential avenues for osteoporosis intervention and treatment.
关键词	Adrenocorticotrophin (ACTH)-secreting pituitary adenoma phenotype genotype osteoporosis
DOI	10.3390/ijms17101654
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[81072084] ; Ministry of Science and Technology of China[2011AA020112] ; Ministry of Science and Technology of China[2014BAI04B00]
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
WOS类目	Biochemistry & Molecular Biology ; Chemistry, Multidisciplinary
WOS记录号	WOS:000387768300128
出版者	MDPI AG
引用统计	被引频次：3[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://119.78.100.204/handle/2XEOYT63/7933
专题	中国科学院计算技术研究所期刊论文_英文
通讯作者	Wang, Renzhi; Zhao, Yi; Jiang, Chengyu
作者单位	1.Chinese Acad Med Sci, Peking Union Med Coll, Peking Union Med Coll Hosp, Dept Neurosurg, Beijing 100730, Peoples R China 2.Chinese Acad Med Sci, Peking Union Med Coll, Peking Union Med Coll Hosp, Ctr Translat Med, Beijing 100730, Peoples R China 3.Cedars Sinai Med Ctr, Joint Pituitary Res Ctr, Beijing 100730, Peoples R China 4.Beijing Union Med Coll Hosp, Beijing 100730, Peoples R China 5.Chinese Acad Med Sci, Inst Basic Med Sci, State Key Lab Med Mol Biol, Beijing 100005, Peoples R China 6.Tsinghua Univ, Peking Union Med Coll, Dept Biochem & Mol Biol, Beijing 100005, Peoples R China 7.Chinese Acad Sci, Key Lab Intelligent Informat Proc, Inst Comp Technol, Beijing 100190, Peoples R China 8.Cedars Sinai Med Ctr, Dept Med, Los Angeles, CA 90048 USA 9.Chinese Acad Med Sci, Peking Union Med Coll, Peking Union Med Coll Hosp, Dept Endocrinol, Beijing 100730, Peoples R China
推荐引用方式 GB/T 7714	Wang, Renzhi,Yang, Yakun,Sheng, Miaomiao,et al. Phenotype-Genotype Association Analysis of ACTH-Secreting Pituitary Adenoma and Its Molecular Link to Patient Osteoporosis[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2016,17(10):16.
APA	Wang, Renzhi.,Yang, Yakun.,Sheng, Miaomiao.,Bu, Dechao.,Huang, Fengming.,...&Jiang, Chengyu.(2016).Phenotype-Genotype Association Analysis of ACTH-Secreting Pituitary Adenoma and Its Molecular Link to Patient Osteoporosis.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,17(10),16.
MLA	Wang, Renzhi,et al."Phenotype-Genotype Association Analysis of ACTH-Secreting Pituitary Adenoma and Its Molecular Link to Patient Osteoporosis".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 17.10(2016):16.