Inhibition of miR-194 suppresses the Wnt/beta-catenin signalling pathway in gastric cancer

doi:10.3892/or.2018.6773

	Inhibition of miR-194 suppresses the Wnt/beta-catenin signalling pathway in gastric cancer
	Peng, Yin 1; Zhang, Xiaojing 1,2; Lin, Huijuan 3,4; Deng, Shiqi 1; Huang, Yong 1; Qin, Ying 5; Feng, Xianling 1; Yan, Ruibin 6; Zhao, Yanqiu 6; Cheng, Yulan 7,8; Wei, Yanjie 9; Wang, Jian 3; Chen, Wangchun 1; Fan, Xinmin 1; Ashktorab, Hassan 10,11; Smoot, Duane 12; Meltzer, Stephen J.7,8; Li, Song 6; Zhang, Zhong 13; Jin, Zhe 1
	2018-12-01
发表期刊	ONCOLOGY REPORTS
ISSN	1021-335X
卷号	40 期号:6 页码:3323-3334
摘要	A mounting body of evidence has revealed that microRNAs (miRs) serve pivotal roles in various developmental processes, and in tumourigenesis, by binding to target genes and subsequently regulating gene expression. Continued activation of the Wnt/-catenin signalling is positively associated with human malignancy. In addition, miR-194 dysregulation has been implicated in gastric cancer (GC); however, the molecular mechanisms underlying the effects of miR-194 on GC carcinogenesis remain to be elucidated. The present study demonstrated that miR-194 was upregulated in GC tissues and SUFU negative regulator of edgehog signaling (SUFU) was downregulated in GC cell lines. Subsequently, inhibition of miR-194 attenuated nuclear accumulation of -catenin, which consequently blocked Wnt/-catenin signalling. In addition, the cytoplasmic translocation of -catenin induced by miR-194 inhibition was mediated by SUFU. Furthermore, genes associated with the Wnt/-catenin signalling pathway were revealed to be downregulated following inhibition of the Wnt signalling pathway by miR-194 suppression. Finally, the results indicated that cell apoptosis was markedly increased in response to miR-194 inhibition, strongly suggesting the carcinogenic effects of miR-194 in GC. Taken together, these findings demonstrated that miR-194 may promote gastric carcinogenesis through activation of the Wnt/-catenin signalling pathway, making it a potential therapeutic target for GC.
关键词	microRNA-194 SUFU negative regulator of edgehog signaling Wnt -catenin apoptosis
DOI	10.3892/or.2018.6773
收录类别	SCI
语种	英语
资助项目	National Nature Science Foundation of China[81772592] ; National Natural Youth Science Foundation of China[31601028] ; Science Technology and Innovation Committee of Shenzhen[JCYJ20170818142852491] ; Science Technology and Innovation Committee of Shenzhen[JCYJ20160331190123578] ; Science Technology and Innovation Committee of Shenzhen[GJHZ20170314154722613] ; Nature Science Foundation of Guangdong Province[2017A030313144] ; Nature Science Foundation of Guangdong Province[2017A030313479] ; Startup Fund of Shenzhen University[2018015] ; Medical Science and Technology Research Foundation of Guangdong Province[A2016112] ; Planned Science and Technology Project of Shenzhen[JCYJ201604221707 22474] ; Guangdong Provincial Department of Science and Technology[2016B090918122] ; NIH[DK087454] ; NIH[CA146799] ; NIH[CA173390] ; American Cancer Society Clinical Research Professorship
WOS研究方向	Oncology
WOS类目	Oncology
WOS记录号	WOS:000447984700017
出版者	SPANDIDOS PUBL LTD
引用统计	被引频次：11[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://119.78.100.204/handle/2XEOYT63/4839
专题	中国科学院计算技术研究所期刊论文_英文
通讯作者	Zhang, Zhong; Jin, Zhe
作者单位	1.Shenzhen Univ, Sch Med, Dept Pathol, Guangdong Key Lab Genome Stabil & Dis Prevent, 3688 Nanhai Ave, Shenzhen 518060, Guangdong, Peoples R China 2.Guangdong Prov Key Lab Mol Oncol Pathol, Dept Pathol, Guangzhou, Guangdong, Peoples R China 3.Guangzhou Med Univ, Dept Pathol & Pathophysiol, Guangzhou, Guangdong, Peoples R China 4.Guangdong Women & Children Hosp, Dept Ultrasound, Guangzhou 510000, Guangdong, Peoples R China 5.Shenzhen Second Peoples Hosp, Dept Gastrointestinal Surg, Shenzhen 518000, Guangdong, Peoples R China 6.Peking Univ, Shenzhen Grad Sch, Lab Chem Genom, Shenzhen 518055, Guangdong, Peoples R China 7.Johns Hopkins Univ, Sch Med, Dept Med, GI Div, Baltimore, MD 21287 USA 8.Sidney Kimmel Comprehens Canc Ctr, Baltimore, MD 21287 USA 9.Shenzhen Inst Adv Technol, Ctr High Performance Comp, Shenzhen 518000, Guangdong, Peoples R China 10.Howard Univ, Coll Med, Dept Med, Washington, DC 20060 USA 11.Howard Univ, Coll Med, Canc Ctr, Washington, DC 20060 USA 12.Meharry Med Ctr, Dept Med, Nashville, TN 37208 USA 13.Shenyang Med Coll, Coll Basic Med Sci, Dept Pathol, 146 Huanghe North St, Shenyang 110034, Liaoning, Peoples R China
推荐引用方式 GB/T 7714	Peng, Yin,Zhang, Xiaojing,Lin, Huijuan,et al. Inhibition of miR-194 suppresses the Wnt/beta-catenin signalling pathway in gastric cancer[J]. ONCOLOGY REPORTS,2018,40(6):3323-3334.
APA	Peng, Yin.,Zhang, Xiaojing.,Lin, Huijuan.,Deng, Shiqi.,Huang, Yong.,...&Jin, Zhe.(2018).Inhibition of miR-194 suppresses the Wnt/beta-catenin signalling pathway in gastric cancer.ONCOLOGY REPORTS,40(6),3323-3334.
MLA	Peng, Yin,et al."Inhibition of miR-194 suppresses the Wnt/beta-catenin signalling pathway in gastric cancer".ONCOLOGY REPORTS 40.6(2018):3323-3334.