Characterization of collision-induced dissociation of deprotonated peptides of 4-16 amino acids using high-resolution mass spectrometry

doi:10.1016/j.ijms.2019.116186

	Characterization of collision-induced dissociation of deprotonated peptides of 4-16 amino acids using high-resolution mass spectrometry
	Zuo, Mei-Qing 1,2,3; Sun, Rui-Xiang 2,3; Fang, Run-Qian 4,5; He, Si-Min 5; Dong, Meng-Qiu 2,3
	2019-11-01
发表期刊	INTERNATIONAL JOURNAL OF MASS SPECTROMETRY
ISSN	1387-3806
卷号	445 页码:8
摘要	Mass spectrometry (MS) analysis of peptides has traditionally been conducted in the positive ion mode on protonated peptides, although negative ion-mode analysis of deprotonated peptides can provide complementary and sometimes critical structural information. This is partly due to insufficient understanding of the fragmentation behaviors of deprotonated peptides. Here, using ion-trap collision-induced dissociation (CID) and higher-energy collisional dissociation (HCD, a beam-type CID), we characterized the fragmentation patterns of 36 deprotonated peptides of 4-16 amino acids on a high-resolution, high-mass accuracy instrument. Our study finds that among the backbone cleavage products, y-, c- and z-type ions (using a nomenclature similar to that of positive ions) are the most dominant species in both CID and HCD spectra of deprotonated peptides, accompanied by abundant neutral loss (NL) peaks. Similar to the charge-dependency of collisional energy of protonated peptides, we find that singly charged deprotonated peptides require significantly higher collisional energy than their doubly or multiply charged counterparts to reach 50% fragmentation. HCD is generally better than CID for peptide sequencing in the negative ion mode, since HCD generates more backbone cleavage products whereas CID produces predominately NL peaks of precursors. For disulfide-bonded peptides and C-terminally amidated peptides, unusual fragmentation is observed in the negative ion mode. The fragmentation behaviors of deprotonated peptides reported in this study will promote further investigation of the fundamental mechanisms and facilitate algorithm development for peptide sequencing in the negative mode. (C) 2019 The Authors. Published by Elsevier B.V.
关键词	Negative-ion mass spectrometry Peptide fragmentation Collision-induced dissociation (CID) Higher-energy collisional dissociation (HCD) Fragmentation efficiency
DOI	10.1016/j.ijms.2019.116186
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China ; Ministry of Science and Technology of China ; Beijing Municipal Science and Technology Commission
WOS研究方向	Physics ; Spectroscopy
WOS类目	Physics, Atomic, Molecular & Chemical ; Spectroscopy
WOS记录号	WOS:000489735800001
出版者	ELSEVIER
引用统计	被引频次：11[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://119.78.100.204/handle/2XEOYT63/4644
专题	中国科学院计算技术研究所期刊论文_英文
通讯作者	Sun, Rui-Xiang; Dong, Meng-Qiu
作者单位	1.China Agr Univ, Coll Biol Sci, Beijing, Peoples R China 2.Natl Inst Biol Sci, Beijing, Peoples R China 3.Tsinghua Univ, Tsinghua Inst Multidisciplinary Biomed Res, Beijing, Peoples R China 4.Univ Chinese Acad Sci, Beijing, Peoples R China 5.Chinese Acad Sci, Inst Comp Technol, Key Lab Intelligent Informat Proc, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Zuo, Mei-Qing,Sun, Rui-Xiang,Fang, Run-Qian,et al. Characterization of collision-induced dissociation of deprotonated peptides of 4-16 amino acids using high-resolution mass spectrometry[J]. INTERNATIONAL JOURNAL OF MASS SPECTROMETRY,2019,445:8.
APA	Zuo, Mei-Qing,Sun, Rui-Xiang,Fang, Run-Qian,He, Si-Min,&Dong, Meng-Qiu.(2019).Characterization of collision-induced dissociation of deprotonated peptides of 4-16 amino acids using high-resolution mass spectrometry.INTERNATIONAL JOURNAL OF MASS SPECTROMETRY,445,8.
MLA	Zuo, Mei-Qing,et al."Characterization of collision-induced dissociation of deprotonated peptides of 4-16 amino acids using high-resolution mass spectrometry".INTERNATIONAL JOURNAL OF MASS SPECTROMETRY 445(2019):8.