Global Quantification of Intact Proteins via Chemical Isotope Labeling and Mass Spectrometry

doi:10.1021/acs.jproteome.9b00071

	Global Quantification of Intact Proteins via Chemical Isotope Labeling and Mass Spectrometry
	Liu, Zheyi 1; Wang, Ruimin 2; Liu, Jing 3; Sun, Ruixiang 2; Wang, Fangjun 1
	2019-05-01
发表期刊	JOURNAL OF PROTEOME RESEARCH
ISSN	1535-3893
卷号	18 期号:5 页码:2185-2194
摘要	Although thousands of intact proteins have been feasibly identified in recent years, global quantification of intact proteins is still challenging. Herein, we develop a high-throughput strategy for global intact protein quantification based on chemical isotope labeling. The isotope incorporation efficiency is as high as 99.2% for complex intact protein samples extracted from HeLa cells. Further, the pTop 2.0 software is developed for automated quantification of intact proteoforms in a high-throughput manner. The high quantification accuracy and reproducibility of this strategy have been demonstrated for both standard and complex cellular protein samples. A total of 2283 intact proteoforms originated from 660 protein accessions are successfully quantified under anaerobic and aerobic conditions and the differentially expressed proteins are observed to be involved in the important biological processes such as stress response.
关键词	mass spectrometry chemical isotope labeling intact protein quantification pTOP highest isotopic peak
DOI	10.1021/acs.jproteome.9b00071
收录类别	SCI
语种	英语
资助项目	National Key R&D Program of China[2016YFF0200504] ; National Natural Science Foundation of China[21675152] ; National Natural Science Foundation of China[91853101] ; National Natural Science Foundation of China[31670837] ; DICP[ZZBS201603]
WOS研究方向	Biochemistry & Molecular Biology
WOS类目	Biochemical Research Methods
WOS记录号	WOS:000467317700023
出版者	AMER CHEMICAL SOC
引用统计	被引频次：13[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://119.78.100.204/handle/2XEOYT63/4250
专题	中国科学院计算技术研究所期刊论文_英文
通讯作者	Sun, Ruixiang; Wang, Fangjun
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Inst Comp Technol, Beijing 100190, Peoples R China 3.Dalian Med Univ, Coll Pharm, Dalian 116044, Peoples R China
推荐引用方式 GB/T 7714	Liu, Zheyi,Wang, Ruimin,Liu, Jing,et al. Global Quantification of Intact Proteins via Chemical Isotope Labeling and Mass Spectrometry[J]. JOURNAL OF PROTEOME RESEARCH,2019,18(5):2185-2194.
APA	Liu, Zheyi,Wang, Ruimin,Liu, Jing,Sun, Ruixiang,&Wang, Fangjun.(2019).Global Quantification of Intact Proteins via Chemical Isotope Labeling and Mass Spectrometry.JOURNAL OF PROTEOME RESEARCH,18(5),2185-2194.
MLA	Liu, Zheyi,et al."Global Quantification of Intact Proteins via Chemical Isotope Labeling and Mass Spectrometry".JOURNAL OF PROTEOME RESEARCH 18.5(2019):2185-2194.