Multiomics Reveals the Mechanism of <i>Natranaerobius thermophilus</i> Adaptation to Combined Hypersaline, Alkaline, and Elevated Temperature Environments

doi:10.1021/acs.jproteome.5c00395

	*Multiomics Reveals the Mechanism of Natranaerobius thermophilus* Adaptation to Combined Hypersaline, Alkaline, and Elevated Temperature Environments**
	Xing, Qinghua 1; Tao, Xinyi 1; Zhang, Shanshan 1,2; Mesbah, Noha M.3; Mao, Xinwei 4; Guo, Xiaomeng 1,5; Hu, Qingping 5; Wang, Haisheng 1; Zhao, Baisuo 1
	2025-08-01
发表期刊	JOURNAL OF PROTEOME RESEARCH
ISSN	1535-3893
卷号	24 期号:8 页码:4243-4258
摘要	The halophilic alkalithermophile N. thermophilus grows optimally at the combined extremes of 3.3-3.9 M Na+, pH 9.5, and 53 degrees C. This study aims to uncover its unique adaptations to the combined extremes of high salt, alkaline pH, and high temperature. Due to the difficulties of genetic manipulation, we used a multiomics approach to reveal the comprehensive transcriptomic, proteomic, and metabolomic landscapes of N. thermophilus under the triple extremes. Specifically, two distinct conditions were evaluated: high salt-alkaline-thermal (HSAT, 4 M Na+/ pH 9.8/52 degrees C) stress and low salt-alkaline-thermal (LSAT, 3 M Na+/ pH 8.8/42 degrees C) stress. Under HSAT stress, N. thermophilus increased the level of saturated fatty acids and uncharged polar lipids to remodel its cell membrane, enhanced Na+-driven flagellar motility, accumulated various compatible solutes, redirected amino acid metabolism for energy, and adjusted the activity of ion transporters and chaperones. These findings exemplify the "No Free Lunch" principle in polyextremophiles. By examining changes in gene, protein, and metabolic regulation levels in N. thermophilus under the simultaneous influence of three extremes, this study provides a comprehensive analysis of the survival mechanisms of polyextremophiles in hypersaline, alkaline, and high-temperature environments. Our findings offer valuable insights into the origins of life on Earth and the potential for extraterrestrial life.
关键词	polyextremophile three-extremes multiomics hypersaline alkaline elevatedtemperature Natranaerobius thermophilus
DOI	10.1021/acs.jproteome.5c00395
收录类别	SCI
语种	英语
资助项目	National Science Foundation of China[31370158] ; National Science Foundation of China[31570110] ; Central Public-interest Scientific Institution Basal Research Fund for Chinese Academy[1610042025005]
WOS研究方向	Biochemistry & Molecular Biology
WOS类目	Biochemical Research Methods
WOS记录号	WOS:001534193700001
出版者	AMER CHEMICAL SOC
引用统计
文献类型	期刊论文
条目标识符	http://119.78.100.204/handle/2XEOYT63/42037
专题	中国科学院计算技术研究所期刊论文_英文
通讯作者	Zhao, Baisuo
作者单位	1.Chinese Acad Agr Sci, Grad Sch, Beijing 100081, Peoples R China 2.Chinese Acad Sci, Inst Comp Technol, Luo Yang Branch, Luoyang 471000, Peoples R China 3.Suez Canal Univ, Fac Pharm, Ismailia 41522, Egypt 4.SUNY Stony Brook, Dept Civil Engn, Stony Brook, NY 11794 USA 5.Shanxi Normal Univ, Coll Life Sci, Linfen 041000, Peoples R China
推荐引用方式 GB/T 7714	Xing, Qinghua,Tao, Xinyi,Zhang, Shanshan,et al. Multiomics Reveals the Mechanism of Natranaerobius thermophilus Adaptation to Combined Hypersaline, Alkaline, and Elevated Temperature Environments[J]. JOURNAL OF PROTEOME RESEARCH,2025,24(8):4243-4258.
APA	Xing, Qinghua.,Tao, Xinyi.,Zhang, Shanshan.,Mesbah, Noha M..,Mao, Xinwei.,...&Zhao, Baisuo.(2025).Multiomics Reveals the Mechanism of Natranaerobius thermophilus Adaptation to Combined Hypersaline, Alkaline, and Elevated Temperature Environments.JOURNAL OF PROTEOME RESEARCH,24(8),4243-4258.
MLA	Xing, Qinghua,et al."Multiomics Reveals the Mechanism of Natranaerobius thermophilus Adaptation to Combined Hypersaline, Alkaline, and Elevated Temperature Environments".JOURNAL OF PROTEOME RESEARCH 24.8(2025):4243-4258.