Enzyme Repertoires and Genomic Insights into Lycium barbarum Pectin Polysaccharide Biosynthesis

doi:10.1093/gpbjnl/qzae079

	Enzyme Repertoires and Genomic Insights into Lycium barbarum Pectin Polysaccharide Biosynthesis
	Yue, Haiyan 1,2; Tang, Yiheng 1,3,4; Li, Aixuan 1,3,5; Zhang, Lili 1,3; Niu, Yiwei 2,3; Zhang, Yiming 1,3; Wang, Hao 1,3; Luo, Jianjun 1,3; Zhao, Yi 6,7; He, Shunmin 1,2; Chen, Chang 3,8; Chen, Runsheng 1,3
	2024-12-01
发表期刊	GENOMICS PROTEOMICS & BIOINFORMATICS
ISSN	1672-0229
卷号	22 期号:6 页码:15
摘要	Lycium barbarum, a member of the Solanaceae family, is an important eudicot with applications in both food and medicine. L. barbarum pectin polysaccharides (LBPPs) are key bioactive compounds of L. barbarum, notable for being among the few polysaccharides with both biocompatibility and biomedical activity. Although studies have analyzed the functional properties of LBPPs, the mechanisms underlying their biosynthesis and transport by key enzymes remain poorly understood. In this study, we assembled a 2.18-Gb reference genome of L. barbarum, reconstructed the first complete biosynthesis pathway of LBPPs, and elucidated the sugar transport system. We also characterized the important genes responsible for backbone extension, sidechain synthesis, and modification of LBPPs. Furthermore, we characterized the long non-coding RNAs (lncRNAs) associated with polysaccharide metabolism. We identified a specific rhamnogalacturonan I (RG-I) rhamnosyltransferase, RRT3020, which enhances RG-I biosynthesis within LBPPs. These newly identified enzymes and pivotal genes endow L. barbarum with unique pectin biosynthesis capabilities, distinguishing it from other Solanaceae species. Our findings thus provide a foundation for evolutionary studies and molecular breeding to expand the diverse applications of L. barbarum.
关键词	Lycium barbarum Lycium barbarum pectin polysaccharide Phylogenetic expansion lncRNA Rhamnogalacturonan I rhamnosyltransferase
DOI	10.1093/gpbjnl/qzae079
收录类别	SCI
语种	英语
资助项目	Ningxia Key Research and Development Program[2016BZ05] ; Ningxia Key Research and Development Program[31701120] ; National Natural Science Foundation of China ; Center for Big Data Research in Health ; Institute of Biophysics, Chinese Academy of Sciences
WOS研究方向	Genetics & Heredity
WOS类目	Genetics & Heredity
WOS记录号	WOS:001470871700001
出版者	OXFORD UNIV PRESS
引用统计
文献类型	期刊论文
条目标识符	http://119.78.100.204/handle/2XEOYT63/40605
专题	中国科学院计算技术研究所期刊论文_英文
通讯作者	Chen, Chang; Chen, Runsheng
作者单位	1.Chinese Acad Sci, Inst Biophys, State Key Lab Epigenet Regulat & Intervent, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Inst Biophys, Ctr Big Data Res Hlth, CAS Key Lab RNA Biol, Beijing 100101, Peoples R China 3.Univ Chinese Acad Sci, Coll Life Sci, Beijing 100049, Peoples R China 4.German Canc Res Ctr, D-69120 Heidelberg, Germany 5.Chinese Univ Hong Kong, Hong Kong 999077, Peoples R China 6.Beijing Univ Chinese Med, Beijing 100029, Peoples R China 7.Chinese Acad Sci, Inst Comp Technol, Adv Comp Res Ctr, Key Lab Intelligent Informat Proc, Beijing 100190, Peoples R China 8.Chinese Acad Sci, Inst Biophys, CAS Ctr Excellence Biomacromolecules, Beijing 100101, Peoples R China
推荐引用方式 GB/T 7714	Yue, Haiyan,Tang, Yiheng,Li, Aixuan,et al. Enzyme Repertoires and Genomic Insights into Lycium barbarum Pectin Polysaccharide Biosynthesis[J]. GENOMICS PROTEOMICS & BIOINFORMATICS,2024,22(6):15.
APA	Yue, Haiyan.,Tang, Yiheng.,Li, Aixuan.,Zhang, Lili.,Niu, Yiwei.,...&Chen, Runsheng.(2024).Enzyme Repertoires and Genomic Insights into Lycium barbarum Pectin Polysaccharide Biosynthesis.GENOMICS PROTEOMICS & BIOINFORMATICS,22(6),15.
MLA	Yue, Haiyan,et al."Enzyme Repertoires and Genomic Insights into Lycium barbarum Pectin Polysaccharide Biosynthesis".GENOMICS PROTEOMICS & BIOINFORMATICS 22.6(2024):15.