Impaired GPX4 activity elicits ferroptosis in alveolar type II cells promoting PHMG-induced pulmonary fibrosis development

doi:10.1016/j.ecoenv.2024.116680

	Impaired GPX4 activity elicits ferroptosis in alveolar type II cells promoting PHMG-induced pulmonary fibrosis development
	Zhang, Wanjun 1,2; Sun, Zhaolong 1,2; Cheng, Wenting 2; Li, Xin 2; Zhang, Jianzhong 2; Li, Yanting 2; Tan, Haining 3; Ji, Xiaoya 2; Zhang, Lin 1,2; Tang, Jinglong 1,2
	2024-08-01
发表期刊	ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
ISSN	0147-6513
卷号	281 页码:13
摘要	Inhaling polyhexamethylene guanidine (PHMG) aerosol, a broad-spectrum disinfectant, can lead to severe pulmonary fibrosis. Ferroptosis, a form of programmed cell death triggered by iron-dependent lipid peroxidation, is believed to play a role in the chemical-induced pulmonary injury. This study aimed to investigate the mechanism of ferroptosis in the progression of PHMG-induced pulmonary fibrosis. C57BL/6 J mice and the alveolar type II cell line MLE-12 were used to evaluate the toxicity of PHMG in vivo and in vitro, respectively. The findings indicated that iron deposition was observed in PHMG induced pulmonary fibrosis mouse model and ferroptosis related genes have changed after 8 weeks PHMG exposure. Additionally, there were disturbances in the antioxidant system and mitochondrial damage in MLE-12 cells following a 12-hour treatment with PHMG. Furthermore, the study observed an increase in lipid peroxidation and a decrease in GPX4 activity in MLE-12 cells after exposure to PHMG. Moreover, pretreatment with the ferroptosis inhibitors Ferrostatin-1 (Fer-1) and Liproxstatin-1 (Lip-1) not only restored the antioxidant system and GPX4 activity but also mitigated lipid peroxidation. Current data exhibit the role of ferroptosis pathway in PHMG-induced pulmonary fibrosis and provide a potential target for future treatment.
关键词	Polyhexamethylene guanidine Pulmonary fibrosis Ferroptosis GPX4 activity Lipid peroxidation
DOI	10.1016/j.ecoenv.2024.116680
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[82273669] ; National Natural Science Foundation of China[82204088] ; National Natural Science Foundation of China[82241086] ; Taishan Scholars Program of Shandong Province ; Innovation and Technology Program for the Excellent Youth Scholars of Higher Education of Shandong Province[2022KJ295]
WOS研究方向	Environmental Sciences & Ecology ; Toxicology
WOS类目	Environmental Sciences ; Toxicology
WOS记录号	WOS:001265323400001
出版者	ACADEMIC PRESS INC ELSEVIER SCIENCE
引用统计	被引频次：1[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://119.78.100.204/handle/2XEOYT63/39861
专题	中国科学院计算技术研究所期刊论文_英文
通讯作者	Zhang, Lin; Tang, Jinglong
作者单位	1.Qingdao Univ, Shandong Prov Maternal & Child Hlth Care Hosp, Jinan 250001, Peoples R China 2.Qingdao Univ, Sch Publ Hlth, Dept Occupat & Environm Hlth, Qingdao 266071, Peoples R China 3.Chinese Acad Sci, Res Ctr Intelligent Comp Syst, Inst Comp Technol, Beijing 100190, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Wanjun,Sun, Zhaolong,Cheng, Wenting,et al. Impaired GPX4 activity elicits ferroptosis in alveolar type II cells promoting PHMG-induced pulmonary fibrosis development[J]. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY,2024,281:13.
APA	Zhang, Wanjun.,Sun, Zhaolong.,Cheng, Wenting.,Li, Xin.,Zhang, Jianzhong.,...&Tang, Jinglong.(2024).Impaired GPX4 activity elicits ferroptosis in alveolar type II cells promoting PHMG-induced pulmonary fibrosis development.ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY,281,13.
MLA	Zhang, Wanjun,et al."Impaired GPX4 activity elicits ferroptosis in alveolar type II cells promoting PHMG-induced pulmonary fibrosis development".ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY 281(2024):13.