Au-Fe3O4 nanozyme coupled with CRISPR-Cas12a for sensitive and visual antibiotic resistance diagnosing

doi:10.1016/j.aca.2023.341014

	Au-Fe3O4 nanozyme coupled with CRISPR-Cas12a for sensitive and visual antibiotic resistance diagnosing
	Chen, Haoxiang 1; Li, Bangying 2; Shi, Shangyi 3,4; Zhou, Tao 1; Wang, Xiumin 2; Wang, Zuyong 5; Zhou, Xi 1; Wang, Miao 1; Shi, Wei 1; Ren, Lei 1,6
	2023-04-22
发表期刊	ANALYTICA CHIMICA ACTA
ISSN	0003-2670
卷号	1251 页码:10
摘要	The accumulation and spread of antibiotic resistance bacteria (ARB) in the environment may accelerate the formation of superbugs and seriously threaten the health of all living beings. The timeliness and accurate diagnosing of antibiotic resistance is essential to controlling the propagation of superbugs in the environment and formulating effective public health management programs. Herein, we developed a speedy, sensitive, ac-curate, and user-friendly colorimetric assay for antibiotic resistance, via a synergistic combination of the peroxidase-like property of the Au-Fe3O4 nanozyme and the specific gene identification capability of the CRISPR-Cas12a. Once the CRISPR-Cas12a system recognizes a target resistance gene, it activates its trans -cleavage activity and subsequently releases the Au-Fe3O4 nanozymes, which oxidizes the 3,3,5,5-tetramethyl-benzidine (TMB) with color change from transparent to blue. The diagnosing signals could be captured and analyzed by a smartphone. This method detected kanamycin-resistance genes, ampicillin-resistance genes, and chloramphenicol-resistance genes by simple operation steps with high sensitivity (<0.1 CFU mu L-1) and speedi-ness (<1 h). This approach may prove easy for the accurate and sensitive diagnosis of the ARGs or ARB in the field, thus surveilling and controlling the microbial water quality flexibly and efficiently.
关键词	CRISPR Cas12a Colorimetric assay Antibiotic resistance bacteria Antibiotic resistance gene Nanozyme Pathogen detection
DOI	10.1016/j.aca.2023.341014
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[31870994] ; National Natural Science Foundation of China[U1904206] ; National Natural Science Foundation of China[22172132] ; Fujian Provincial Science and Technology Cooperation Project[20210002] ; Natural Science Foundation of Xiamen, China[3502Z20227010]
WOS研究方向	Chemistry
WOS类目	Chemistry, Analytical
WOS记录号	WOS:000990752700001
出版者	ELSEVIER
引用统计	被引频次：11[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://119.78.100.204/handle/2XEOYT63/21420
专题	中国科学院计算技术研究所期刊论文_英文
通讯作者	Shi, Wei; Ren, Lei
作者单位	1.Xiamen Univ, Coll Mat, Res Ctr Biomed Engn Xiamen, Dept Biomat,Higher Educ Key Lab Biomed Engn Fujian, Xiamen 361005, Peoples R China 2.Xiamen Univ, Sch Pharmaceut Sci, Xiamen 361102, Peoples R China 3.Chinese Acad Sci, Inst Comp Technol, State Key Lab Comp Architecture, Beijing 100190, Peoples R China 4.Univ Chinese Acad Sci, Sch Comp Sci & Technol, Beijing 100049, Peoples R China 5.Hunan Univ, Coll Mat Sci & Engn, Changsha 410072, Peoples R China 6.Xiamen Univ, State Key Lab Phys Chem Solid Surfaces, Xiamen 361005, Peoples R China
推荐引用方式 GB/T 7714	Chen, Haoxiang,Li, Bangying,Shi, Shangyi,et al. Au-Fe3O4 nanozyme coupled with CRISPR-Cas12a for sensitive and visual antibiotic resistance diagnosing[J]. ANALYTICA CHIMICA ACTA,2023,1251:10.
APA	Chen, Haoxiang.,Li, Bangying.,Shi, Shangyi.,Zhou, Tao.,Wang, Xiumin.,...&Ren, Lei.(2023).Au-Fe3O4 nanozyme coupled with CRISPR-Cas12a for sensitive and visual antibiotic resistance diagnosing.ANALYTICA CHIMICA ACTA,1251,10.
MLA	Chen, Haoxiang,et al."Au-Fe3O4 nanozyme coupled with CRISPR-Cas12a for sensitive and visual antibiotic resistance diagnosing".ANALYTICA CHIMICA ACTA 1251(2023):10.