Click-iG: Simultaneous Enrichment and Profiling of Intact N-linked, O-GalNAc, and O-GlcNAcylated Glycopeptides

doi:10.1002/anie.202303410

	Click-iG: Simultaneous Enrichment and Profiling of Intact N-linked, O-GalNAc, and O-GlcNAcylated Glycopeptides
	Liu, Jialin 1,2,3,4; Cheng, Bo 1,2; Fan, Xinqi 1,2; Zhou, Xinyue 1,2; Wang, Jiankun 1,2; Zhou, Wen 1,2; Li, Hengyu 1,2; Zeng, Wenfeng 5,6; Yang, Pengyuan 3,4; Chen, Xing 1,2
	2023-07-25
发表期刊	ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
ISSN	1433-7851
页码	12
摘要	Proteins are ubiquitously modified with glycans of varied chemical structures through distinct glycosidic linkages, making the landscape of protein glycosylation challenging to map. Profiling of intact glycopeptides with mass spectrometry (MS) has recently emerged as a powerful tool for revealing matched information of the glycosylation sites and attached glycans (i.e., intact glycosites), but is largely limited to individual glycosylation types. Herein, we describe Click-iG, which integrates metabolic labeling of glycans with clickable unnatural sugars, an optimized MS method, and a tailored version of pGlyco3 software to enable simultaneous enrichment and profiling of three types of intact glycopeptides: N-linked, mucin-type O-linked, and O-GlcNAcylated glycopeptides. We demonstrate the utility of Click-iG by the identification of thousands of intact glycosites in cell lines and living mice. From the mouse lung, heart, and spleen, a total of 2053 intact N-glycosites, 262 intact O-GalNAc glycosites, and 1947 O-GlcNAcylation sites were identified. Click-iG-enabled comprehensive coverage of the protein glycosylation landscape lays the foundation for interrogating crosstalk between different glycosylation pathways.
关键词	Glycoproteomics Glycosylation Intact Glycosites Metabolic Labeling Unnatural Glycans
DOI	10.1002/anie.202303410
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[92153301] ; National Natural Science Foundation of China[2225000285] ; National Natural Science Foundation of China[22037001] ; National Key Ramp;D Program of China[2018YFA0507600]
WOS研究方向	Chemistry
WOS类目	Chemistry, Multidisciplinary
WOS记录号	WOS:001035289900001
出版者	WILEY-V C H VERLAG GMBH
引用统计	被引频次：2[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://119.78.100.204/handle/2XEOYT63/21280
专题	中国科学院计算技术研究所期刊论文_英文
通讯作者	Chen, Xing
作者单位	1.Peking Univ, Coll Chem & Mol Engn, Peking Tsinghua Ctr Life Sci, Synthet & Funct Biomol Ctr,Beijing Natl Lab Mol Sc, Beijing 100871, Peoples R China 2.Peking Univ, Key Lab Bioorgan Chem & Mol Engn, Minist Educ, Beijing 100871, Peoples R China 3.Fudan Univ, Inst Biomed Sci, Shanghai 200433, Peoples R China 4.Fudan Univ, Dept Chem, Shanghai 200433, Peoples R China 5.Chinese Acad Sci, Key Lab Intelligent Informat Proc, Beijing 100190, Peoples R China 6.Chinese Acad Sci, Inst Comp Technol, Beijing 100190, Peoples R China
推荐引用方式 GB/T 7714	Liu, Jialin,Cheng, Bo,Fan, Xinqi,et al. Click-iG: Simultaneous Enrichment and Profiling of Intact N-linked, O-GalNAc, and O-GlcNAcylated Glycopeptides[J]. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION,2023:12.
APA	Liu, Jialin.,Cheng, Bo.,Fan, Xinqi.,Zhou, Xinyue.,Wang, Jiankun.,...&Chen, Xing.(2023).Click-iG: Simultaneous Enrichment and Profiling of Intact N-linked, O-GalNAc, and O-GlcNAcylated Glycopeptides.ANGEWANDTE CHEMIE-INTERNATIONAL EDITION,12.
MLA	Liu, Jialin,et al."Click-iG: Simultaneous Enrichment and Profiling of Intact N-linked, O-GalNAc, and O-GlcNAcylated Glycopeptides".ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2023):12.