Determining protein structures in cellular lamella at pseudo-atomic resolution by GisSPA

doi:10.1038/s41467-023-36175-y

	Determining protein structures in cellular lamella at pseudo-atomic resolution by GisSPA
	Cheng, Jing 1; Liu, Tong 2; You, Xin 3; Zhang, Fa 4; Sui, Sen-Fang 3; Wan, Xiaohua 4; Zhang, Xinzheng 1
	2023-03-15
发表期刊	NATURE COMMUNICATIONS
卷号	14 期号:1 页码:9
摘要	Cryo-electron tomography is a major tool used to study the structure of protein complexes in situ. However, the throughput of tilt-series image data collection is still quite low. Here, we show that GisSPA, a GPU accelerated program, can translationally and rotationally localize the target protein complex in cellular lamellae, as prepared with a focused ion beam, using single cryo-electron microscopy images without tilt-series, and reconstruct the protein complex at near-atomic resolution. GisSPA allows high-throughput data collection without the acquisition of tilt-series images and reconstruction of the tomogram, which is essential for high-resolution reconstruction of asymmetric or low-symmetry protein complexes. We demonstrate the power of GisSPA with 3.4-angstrom and 3.9-angstrom resolutions of resolving phycobilisome and tetrameric photosystem II complex structures in cellular lamellae, respectively. In this work, we present GisSPA as a practical tool that facilitates high-resolution in situ protein structure determination. High-resolution in situ protein structure can be solved by cryo-ET, which requires several days of data collection. Here Cheng et al. report GisSPA, a program that may enable determining sub-4 angstrom resolution structures on cellular lamellae within one day of data collection.
DOI	10.1038/s41467-023-36175-y
收录类别	SCI
语种	英语
资助项目	National Key R&D Program of China[2021YFA1301501] ; National Key R&D Program of China[2017YFA0504700] ; National Natural Science Foundation of China[31930069] ; National Natural Science Foundation of China[32150010] ; National Natural Science Foundation of China[61932018] ; National Natural Science Foundation of China[32241027] ; National Natural Science Foundation of China[62072441] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDB37040101] ; Key Research Program of Frontier Sciences at the Chinese Academy of Sciences[ZDBS-LY-SM003]
WOS研究方向	Science & Technology - Other Topics
WOS类目	Multidisciplinary Sciences
WOS记录号	WOS:001001760400005
出版者	NATURE PORTFOLIO
引用统计	被引频次：7[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://119.78.100.204/handle/2XEOYT63/21190
专题	中国科学院计算技术研究所期刊论文_英文
通讯作者	Wan, Xiaohua; Zhang, Xinzheng
作者单位	1.Chinese Acad Sci, Inst Biophys, CAS Ctr Excellence Biomacromolecules, Natl Lab Biomacromolecules, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Inst Comp Technol, High Performance Comp Res Ctr, Beijing 100190, Peoples R China 3.Tsinghua Univ, Beijing Adv Innovat Ctr Struct Biol, Sch Life Sci, State Key Lab Membrane Biol, Beijing 100084, Peoples R China 4.Beijing Inst Technol, Beijing 100081, Peoples R China
推荐引用方式 GB/T 7714	Cheng, Jing,Liu, Tong,You, Xin,et al. Determining protein structures in cellular lamella at pseudo-atomic resolution by GisSPA[J]. NATURE COMMUNICATIONS,2023,14(1):9.
APA	Cheng, Jing.,Liu, Tong.,You, Xin.,Zhang, Fa.,Sui, Sen-Fang.,...&Zhang, Xinzheng.(2023).Determining protein structures in cellular lamella at pseudo-atomic resolution by GisSPA.NATURE COMMUNICATIONS,14(1),9.
MLA	Cheng, Jing,et al."Determining protein structures in cellular lamella at pseudo-atomic resolution by GisSPA".NATURE COMMUNICATIONS 14.1(2023):9.