Improved localization precision via restricting confined biomolecule stochastic motion in singlemolecule localization microscopy

doi:10.1515/nanoph-2021-0481

	Improved localization precision via restricting confined biomolecule stochastic motion in singlemolecule localization microscopy
	Ni, Jielei 1,2; Cao, Bo 1,2,3; Niu, Gang 4,5,6,7,8; Chen, Danni 1,2,3; Liang, Guotao 1,2; Xia, Tingying 1,2; Li, Heng 9; Xu, Chen 1,2; Wang, Jingyu 1,2; Zhang, Wanlong 1,2; Zhang, Yilin 1,2; Yuan, Xiaocong 1,2; Ni, Yanxiang 1,2
	2022
发表期刊	NANOPHOTONICS
ISSN	2192-8606
卷号	11 期号:1 页码:53-65
摘要	Single-molecule localization microscopy (SMLM) plays an irreplaceable role in biological studies, in which nanometer-sized biomolecules are hardly to be resolved due to diffraction limit unless being stochastically activated and accurately located by SMLM. For biological samples preimmobilized for SMLM, most biomolecules are cross-linked and constrained at their immobilizing sites but still expected to undergo confined stochastic motion in regard to their nanometer sizes. However, few lines of direct evidence have been reported about the detectability and influence of confined biomolecule stochastic motion on localization precision in SMLM. Here, we access the potential stochastic motion for each immobilized single biomolecule by calculating the displacements between any two of its localizations at different frames during sequential imaging of Alexa Fluor647-conjugated oligonucleotides. For most molecules, localization displacements are remarkably larger at random frame intervals than at shortest intervals even after sample drift correction, increase with interval times and then saturate, showing that biomolecule stochastic motion is detected and confined around the immobilizing sizes in SMLM. Moreover, localization precision is inversely proportional to confined biomolecule stochasticmotion, whereas it can be deteriorated or improved by enlarging the biomolecules or adding a postcrosslinking step, respectively. Consistently, post-crosslinking of cell samples sparsely stained for tubulin proteins results in a better localization precision. Overall, this study reveals that confined stochastic motion of immobilized biomolecules worsens localization precision in SMLM, and improved localization precision can be achieved via restricting such a motion.
关键词	confined stochastic motion localization precision single-molecule localization microscopy
DOI	10.1515/nanoph-2021-0481
收录类别	SCI
语种	英语
资助项目	Guangdong Major Project of Basic and Applied Basic Research[2020B0301030009] ; National Natural Science Foundation of China[31871293] ; National Natural Science Foundation of China[11774242] ; National Natural Science Foundation of China[62005180] ; National Natural Science Foundation of China[62105212] ; Shenzhen Science and Technology Planning Project[JCYJ202103240 93209024] ; Shenzhen Science and Technology Planning Project[JCYJ20210324094200001] ; Shenzhen Science and Technology Planning Project[JCYJ20170817095211560] ; Shenzhen Peacock Plan[KQTD20170330110444030] ; Natural Science Foundation of Guangdong Province[2016A030312010] ; leading talents of Guangdong province[00201505] ; Hefei National Laboratory for Physical Sciences at the Microscale[KF2020009]
WOS研究方向	Science & Technology - Other Topics ; Materials Science ; Optics ; Physics
WOS类目	Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary ; Optics ; Physics, Applied
WOS记录号	WOS:000865440400005
出版者	WALTER DE GRUYTER GMBH
引用统计	被引频次：3[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://119.78.100.204/handle/2XEOYT63/19783
专题	中国科学院计算技术研究所期刊论文
通讯作者	Ni, Yanxiang
作者单位	1.Shenzhen Univ, Coll Phys & Optoelect Engn, Coll Elect & Informat Engn, Nanophoton Res Ctr,Shenzhen Key Lab Microscale Op, Shenzhen 518060, Peoples R China 2.Shenzhen Univ, Coll Phys & Optoelect Engn, Coll Elect & Informat Engn, Inst Microscale Optoelect, Shenzhen 518060, Peoples R China 3.Dr Neon Technol Ltd, Shenzhen 518060, Peoples R China 4.Phil Rivers Technol, Beijing 100871, Peoples R China 5.Phil Rivers Technol Ltd, Joint Turing Darwin Lab, Beijing 100190, Peoples R China 6.Chinese Acad Sci, Inst Comp Technol, Beijing 100190, Peoples R China 7.Chinese Acad Sci, Western Inst Comp Technol, Chongqing 400000, Peoples R China 8.Univ Sci & Technol China, Sch Basic Med Sci, Div Life Sci & Med, Hefei Natl Lab Phys Sci Microscale, Hefei 231299, Peoples R China 9.Tsinghua Univ, Tsinghua Berkeley Shenzhen Inst TBSI, Shenzhen 518055, Peoples R China
推荐引用方式 GB/T 7714	Ni, Jielei,Cao, Bo,Niu, Gang,et al. Improved localization precision via restricting confined biomolecule stochastic motion in singlemolecule localization microscopy[J]. NANOPHOTONICS,2022,11(1):53-65.
APA	Ni, Jielei.,Cao, Bo.,Niu, Gang.,Chen, Danni.,Liang, Guotao.,...&Ni, Yanxiang.(2022).Improved localization precision via restricting confined biomolecule stochastic motion in singlemolecule localization microscopy.NANOPHOTONICS,11(1),53-65.
MLA	Ni, Jielei,et al."Improved localization precision via restricting confined biomolecule stochastic motion in singlemolecule localization microscopy".NANOPHOTONICS 11.1(2022):53-65.