Discovery of the correlation between the suspended membrane capacitance and adherent morphology of single cells enriching from clinical pleural effusion revealed by a microfluidic impedance flow cytometry

doi:10.1016/j.snb.2022.132487

	Discovery of the correlation between the suspended membrane capacitance and adherent morphology of single cells enriching from clinical pleural effusion revealed by a microfluidic impedance flow cytometry
	Luan, Xiaofeng 1,2; Li, Yuang 1,2; Zhao, Haiping 3; Sun, Sheng 1,2; Fan, Yuanyuan 1; Zhang, Wenchang 1; Zhang, Lingqian 1; Li, Mingxiao 1; Wang, Jinghui 4; Zhi, Tian 5; Zhang, Lina 6; Zhao, Yang 1; Huang, Chengjun 1,2
	2022-11-15
发表期刊	SENSORS AND ACTUATORS B-CHEMICAL
卷号	371 页码:11
摘要	Cellular adherent morphology and suspended electrical property are two important intrinsic biophysical features of single cells in two states. However, few studies reported their relationship due to lacking systematic methods. Here, we proposed a toolchain for enriching, proliferating single cells from pleural effusions (PEs), and characterizing their adherent morphologies and suspended inherent electrical properties. Our 3D cell sieving device was employed to enrich rare tumor cells from every 50 mL clinical PEs. After proliferated, ten samples were enrolled, whose cells' adherent morphologies were quantified with the elongation ratio (Er). Our microfluidic impedance flow cytometry was developed to characterize similar to 65,400 suspended single cells' electrical properties (e.g., C-sm). Subsequently, we experimentally found that the C-sm of 5 spindle-like (mainly Er> 2) samples were all quantified as focused above 1.5 mu F/cm(2), whereas others' were all focused around 1-1.5 mu F/cm(2) for 5 round-like (mainly Er= 2) samples. Spearman rhos were introduced to further quantify this potential correlation from aspects of proportions (C-sm> 1.5 mu F/cm(2), Er> 2), average, and median, noting as 0.758 (p = 0.011), 0.760 (p = 0.011), and 0.744 (p = 0.014), respectively. Those results revealed a significant correlation between single cells' Er and C-sm-which means that the underlying correlation between cells' two label-free biophysical properties presented in two states was discovered.
关键词	Pleural effusion (PE) 3D cell sieving Cell adherent morphology Elongation ratio Single -cell electrical properties Specific membrane capacitance
DOI	10.1016/j.snb.2022.132487
收录类别	SCI
语种	英语
资助项目	National Key Research and Devel-opment Program of China[2018YFC2001100] ; National Natural Science Foundation of China[62171441] ; Scientific Research and Equipment Development Project of CAS[YJKYYQ20210031] ; State Key Laboratory of Computer Architecture (ICT, CAS)[CARCH202122] ; Youth Innovation Promotion Association of ChineseAcademy of Sciences
WOS研究方向	Chemistry ; Electrochemistry ; Instruments & Instrumentation
WOS类目	Chemistry, Analytical ; Electrochemistry ; Instruments & Instrumentation
WOS记录号	WOS:000843505600005
出版者	ELSEVIER SCIENCE SA
引用统计	被引频次：5[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://119.78.100.204/handle/2XEOYT63/19465
专题	中国科学院计算技术研究所期刊论文_英文
通讯作者	Zhang, Lina; Zhao, Yang; Huang, Chengjun
作者单位	1.Chinese Acad Sci, Inst Microelect, Beijing 100029, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Xuanwu Hosp Capital Med Univ, Cerebrovascular Dis Res Inst, Beijing 100053, Peoples R China 4.Capital Med Univ, Beijing Chest Hosp, Beijing TB & Thorac Tumor Res Inst, Dept Med Oncol, Beijing 101125, Peoples R China 5.Chinese Acad Sci, Inst Comp Technol, State Key Lab Comp Architecture, Beijing 101125, Peoples R China 6.Capital Med Univ, Beijing Chest Hosp, Beijing TB & Thorac Tumor Res Inst, Canc Res Ctr, Beijing 101125, Peoples R China
推荐引用方式 GB/T 7714	Luan, Xiaofeng,Li, Yuang,Zhao, Haiping,et al. Discovery of the correlation between the suspended membrane capacitance and adherent morphology of single cells enriching from clinical pleural effusion revealed by a microfluidic impedance flow cytometry[J]. SENSORS AND ACTUATORS B-CHEMICAL,2022,371:11.
APA	Luan, Xiaofeng.,Li, Yuang.,Zhao, Haiping.,Sun, Sheng.,Fan, Yuanyuan.,...&Huang, Chengjun.(2022).Discovery of the correlation between the suspended membrane capacitance and adherent morphology of single cells enriching from clinical pleural effusion revealed by a microfluidic impedance flow cytometry.SENSORS AND ACTUATORS B-CHEMICAL,371,11.
MLA	Luan, Xiaofeng,et al."Discovery of the correlation between the suspended membrane capacitance and adherent morphology of single cells enriching from clinical pleural effusion revealed by a microfluidic impedance flow cytometry".SENSORS AND ACTUATORS B-CHEMICAL 371(2022):11.