O-GlcNAcylation Quantification Based on Electron-Transfer/Higher-Energy Collisional Dissociation and Its Application in O-GlcNAcylation Site Mapping of Liver Proteomics in High Fat Diet-fed Mice

doi:10.19756/j.issn.0253-3820.210623

	O-GlcNAcylation Quantification Based on Electron-Transfer/Higher-Energy Collisional Dissociation and Its Application in O-GlcNAcylation Site Mapping of Liver Proteomics in High Fat Diet-fed Mice
	Shao Wen-Ya 1,2; Liang Yu 2; Liu Jian-Xi 4; Liu Hong-Tao 3; Wang Zhao-Wei 5; Sui Zhi-Gang 2; Zhao Bao-Feng 2; Zhang Xiao-Dan 2; Liang Zhen 2; Zhang Li-Hua 2; Zhang Yu-Kui 2
	2021-12-01
发表期刊	CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
ISSN	0253-3820
卷号	49 期号:12 页码:11
摘要	O-linked beta-N-acetylglucosaminylation (O-GlcNAcylation ) is a single carbohydrate moiety post-translational modification that occurs on serine and threonine side chains of intracellular protein and plays an important role in nutrient metabolism. However, the great research challenges are the inherently low stoichiometry, , poor ionization efficiency of O-GlcNAc peptides and no specific animo acid sequence. In this study, a strategy for quantitative detection of O-GlcNAc sites was developed by combining pseudo-isobaric dimethyl strategy, which was applied for the O-GlcNAcylation sites profiling towards the mice liver. In total, 783 O-GlcNAc sites were unambiguously quantified from high-fat fed mice liver. Among which , 122 O-GlcNAc sites were differentially expressed, corresponding to 85 O-GlcNAc proteins. Finally, the biological function of the differentially expressed O-GlcNAc proteins was analyzed.
关键词	O-linked beta-N-acetylglucosaminylation Quantitative proteomics Electron transfer/higher energy collision dissociation
DOI	10.19756/j.issn.0253-3820.210623
收录类别	SCI
语种	英语
WOS研究方向	Chemistry
WOS类目	Chemistry, Analytical
WOS记录号	WOS:000753629000001
出版者	SCIENCE PRESS
引用统计
文献类型	期刊论文
条目标识符	http://119.78.100.204/handle/2XEOYT63/18997
专题	中国科学院计算技术研究所期刊论文_英文
通讯作者	Zhang Li-Hua
作者单位	1.Fujian Med Univ, Sch Publ Hlth, Dept Prevent Med, Fuzhou 350122, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China 3.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China 4.Fujian Normal Univ, Coll Environm Sci & Engn, Fuzhou 350007, Peoples R China 5.Chinese Acad Sci, Inst Comp Technol, Beijing 100190, Peoples R China
推荐引用方式 GB/T 7714	Shao Wen-Ya,Liang Yu,Liu Jian-Xi,et al. O-GlcNAcylation Quantification Based on Electron-Transfer/Higher-Energy Collisional Dissociation and Its Application in O-GlcNAcylation Site Mapping of Liver Proteomics in High Fat Diet-fed Mice[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2021,49(12):11.
APA	Shao Wen-Ya.,Liang Yu.,Liu Jian-Xi.,Liu Hong-Tao.,Wang Zhao-Wei.,...&Zhang Yu-Kui.(2021).O-GlcNAcylation Quantification Based on Electron-Transfer/Higher-Energy Collisional Dissociation and Its Application in O-GlcNAcylation Site Mapping of Liver Proteomics in High Fat Diet-fed Mice.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,49(12),11.
MLA	Shao Wen-Ya,et al."O-GlcNAcylation Quantification Based on Electron-Transfer/Higher-Energy Collisional Dissociation and Its Application in O-GlcNAcylation Site Mapping of Liver Proteomics in High Fat Diet-fed Mice".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 49.12(2021):11.