The effect of N-glycosylation of SARS-CoV-2 spike protein on the virus interaction with the host cell ACE2 receptor

doi:10.1016/j.isci.2021.103272

	The effect of N-glycosylation of SARS-CoV-2 spike protein on the virus interaction with the host cell ACE2 receptor
	Huang, Chuncui 1,5; Tan, Zeshun 1,3; Zhao, Keli 1,3; Zou, Wenjun 1,3; Wang, Hui 2,3; Gao, Huanyu 1; Sun, Shiwei 2,3; Bu, Dongbo 2,3; Chai, Wengang 4; Li, Yan 1,3,5
	2021-11-19
发表期刊	ISCIENCE
卷号	24 期号:11 页码:15
摘要	The densely glycosylated spike (S) protein highly exposed on severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) surface mediates host cell entry by binding to the receptor angiotensin-converting enzyme 2 (ACE2). However, the role of glycosylation has not been fully understood. In this study, we investigated the effect of different N-glycosylation of S1 protein on its binding to ACE2. Using real-time surface plar non resonance assay the negative effects were demonstrated by the considerable increase of binding affirities of de-Nglycosylated Si proteins produced from three different expression systems including baculovirus-insect, Chinese hamster ovarian and two variants of human embryonic kidney 293 cells. Molecular dynamic simulations of the S1 protein-ACE2 receptor complex revealed the steric hindrance and Coulombic repulsion effects of different types of N-glycans on the S1 protein interaction with ACE2. The results should contribute to future pathological studies of SARS-CoV-2 and therapeutic development of Covid-19, particularly using recombinant Si proteins as models.
DOI	10.1016/j.isci.2021.103272
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[31600650] ; National Natural Science Foundation of China[31671369] ; National Natural Science Foundation of China[62072435] ; Biological Resources Program, Chinese Academy of Sciences[KFJ-BRP-017-76] ; March of Dimes Prematurity Research Center grant[22-FY18-82] ; Wellcome Trust Biomedical Resource grant[218304/Z/19/Z] ; project of Dengfeng Planfrom Foshan Hospi-tal of Traditional Chinese Medicine
WOS研究方向	Science & Technology - Other Topics
WOS类目	Multidisciplinary Sciences
WOS记录号	WOS:000730105700009
出版者	CELL PRESS
引用统计	被引频次：23[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://119.78.100.204/handle/2XEOYT63/18403
专题	中国科学院计算技术研究所期刊论文_英文
通讯作者	Chai, Wengang; Li, Yan
作者单位	1.Chinese Acad Sci, Inst Biophys, 15 Datun Rd, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Inst Comp Technol, Key Lab Intelligent Informat Proc, 6 Kexueyuan South Rd, Beijing 100080, Peoples R China 3.Univ Chinese Acad Sci, 19 Yuquan Rd, Beijing 100049, Peoples R China 4.Imperial Coll London, Fac Med, Glycosci Lab, London W12 0NN, England 5.Guangzhou Regenerat Med & Hlth Guangdong Lab, Bioland Lab, Guangzhou 510005, Peoples R China
推荐引用方式 GB/T 7714	Huang, Chuncui,Tan, Zeshun,Zhao, Keli,et al. The effect of N-glycosylation of SARS-CoV-2 spike protein on the virus interaction with the host cell ACE2 receptor[J]. ISCIENCE,2021,24(11):15.
APA	Huang, Chuncui.,Tan, Zeshun.,Zhao, Keli.,Zou, Wenjun.,Wang, Hui.,...&Li, Yan.(2021).The effect of N-glycosylation of SARS-CoV-2 spike protein on the virus interaction with the host cell ACE2 receptor.ISCIENCE,24(11),15.
MLA	Huang, Chuncui,et al."The effect of N-glycosylation of SARS-CoV-2 spike protein on the virus interaction with the host cell ACE2 receptor".ISCIENCE 24.11(2021):15.