Radiosensitivity-Specific Proteomic and Signaling Pathway Network of Non-Small Cell Lung Cancer (NSCLC)

doi:10.1016/j.ijrobp.2021.08.041

	Radiosensitivity-Specific Proteomic and Signaling Pathway Network of Non-Small Cell Lung Cancer (NSCLC)
	Zhu, Xueru 1; Wang, Yiting 1; Jiang, Chang 1; Li, Xiaoyang 2; Sun, Linying 3; Wang, Guangzhong 3; Fu, Xiaolong 1
	2022-02-01
发表期刊	INTERNATIONAL JOURNAL OF RADIATION ONCOLOGY BIOLOGY PHYSICS
ISSN	0360-3016
卷号	112 期号:2 页码:529-541
摘要	Purpose: An unmet clinical need in non-small cell lung cancer (NSCLC) management is the accurate prediction of radiation response in patients receiving radical radiation therapy. We explored the intrinsic radiosensitivity of NSCLC from the proteomic profiles of NSCLC cell lines and paraffin-embedded human samples. Methods and Materials: To uncover radiosensitivity-specific proteomic and signaling pathways, we performed quantitative proteomics by data-independent acquisition mass spectrometry assay on 29 human NSCLC cell lines and 13 paraffin-embedded human NSCLC samples. We validated closely interacting radioresistant proteins by western blotting, immunofluorescence, real-time quantitative polymerase chain reaction in NSCLC cell lines, and immunohistochemistry in paraffinembedded human samples. We validated the functions of 3 key hub proteins by lentivirus transfection, clonogenic survival assay, and flow cytometry. Results: The proteomic profiling of NSCLC showed that the intrinsic radiosensitivity of NSCLC is mainly modulated by signaling pathways of proteoglycans in cancer, focal adhesion, and regulation of the actin cytoskeleton. We identified 71 differentially expressed proteins and validated 8 closely interacting proteins as radioresistant proteins of NSCLC. Moreover, we also validated the functionality of integrin-linked protein kinase, p21-activated kinase 1, and Ras GTPase-activating-like protein IQGAP1 in the radiation response of NSCLC cell lines. Finally, with the NSCLC radiosensitivity-specific proteins, we delineated the atlas network of NSCLC radiosensitivity-related signaling pathways. Conclusions: Radiosensitivity-specific proteins could guide individualized radiation therapy in clinical practice by predicting the radiation response of patients with NSCLC. Moreover, the NSCLC radiosensitivity-related signaling pathway atlas could guide further exploration of the underlying mechanism. (C) 2021 Elsevier Inc. All rights reserved.
DOI	10.1016/j.ijrobp.2021.08.041
收录类别	SCI
语种	英语
资助项目	Shanghai Chest Hospital Project of Collaborative Innovation[YJXT20190101] ; National Natural Science Foundation of China[92059206]
WOS研究方向	Oncology ; Radiology, Nuclear Medicine & Medical Imaging
WOS类目	Oncology ; Radiology, Nuclear Medicine & Medical Imaging
WOS记录号	WOS:000740708800066
出版者	ELSEVIER SCIENCE INC
引用统计	被引频次：8[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://119.78.100.204/handle/2XEOYT63/18278
专题	中国科学院计算技术研究所期刊论文_英文
通讯作者	Fu, Xiaolong
作者单位	1.Shanghai Jiao Tong Univ, Shanghai Chest Hosp, Dept Radiat Oncol, Shanghai, Peoples R China 2.Univ Sci & Technol China, Affiliated Hosp 1, Dept Radiat Oncol, Hefei, Anhui, Peoples R China 3.Chinese Acad Sci, Inst Comp Technol, Shanghai, Peoples R China
推荐引用方式 GB/T 7714	Zhu, Xueru,Wang, Yiting,Jiang, Chang,et al. Radiosensitivity-Specific Proteomic and Signaling Pathway Network of Non-Small Cell Lung Cancer (NSCLC)[J]. INTERNATIONAL JOURNAL OF RADIATION ONCOLOGY BIOLOGY PHYSICS,2022,112(2):529-541.
APA	Zhu, Xueru.,Wang, Yiting.,Jiang, Chang.,Li, Xiaoyang.,Sun, Linying.,...&Fu, Xiaolong.(2022).Radiosensitivity-Specific Proteomic and Signaling Pathway Network of Non-Small Cell Lung Cancer (NSCLC).INTERNATIONAL JOURNAL OF RADIATION ONCOLOGY BIOLOGY PHYSICS,112(2),529-541.
MLA	Zhu, Xueru,et al."Radiosensitivity-Specific Proteomic and Signaling Pathway Network of Non-Small Cell Lung Cancer (NSCLC)".INTERNATIONAL JOURNAL OF RADIATION ONCOLOGY BIOLOGY PHYSICS 112.2(2022):529-541.