Systematic Survey of the Alteration of the Faecal Microbiota in Rats With Gastrointestinal Disorder and Modulation by Multicomponent Drugs

doi:10.3389/fphar.2021.670335

	Systematic Survey of the Alteration of the Faecal Microbiota in Rats With Gastrointestinal Disorder and Modulation by Multicomponent Drugs
	Wu, Yue 1,2; Wu, Yang 2,3; Wu, Hongwei 1; Wu, Changxun 1; Ji, Enhui 1; Xu, Jing 1; Zhang, Yi 1; Wei, Junying 1; Zhao, Yi 3; Yang, Hongjun 2
	2021-11-02
发表期刊	FRONTIERS IN PHARMACOLOGY
卷号	12 页码:13
摘要	Gastrointestinal disorder (GID) is a global health disease which leads to heavy public medical burden. Disorders in the intestinal flora have been found in gastrointestinal disorder patients. However, the interaction between GID and the intestinal flora in faecal has not been studied comprehensively. In addition, multicomponent drugs represented by traditional Chinese medicine (TCM) are widely used for treating GID, but their modulation of the intestinal flora has not been investigated. Therefore, in this study, a high-throughput sequencing strategy was used to investigate alterations in the intestinal flora in a rat GID model, followed by an investigation of the modulation by a representative TCM, Xiaoerfupi (XEFP) granule. The results showed that in rats with GID, the relative abundances of Erysipelotrichaceae, Lachnospiraceae, Streptococcaceae increased and that of Ruminococcaceae decreased. At the macro level, the levels of LysoPC(16:0), LysoPC(20:2), LysoPC(15:0), LysoPC(20:2 (11Z, 14Z)), LysoPC(20:1), LysoPC(15:0), LysoPC(20:0) and LysoPE (0:0/20:0) in serum increased and levels of PC(36:4), PC(38:4), PC(o-36;4), PE (MonoMe(13,5)/MonoMe(11,5)) decreased. The imbalance of metabolites was restored by XEFP through ether lipid metabolism pathway. Increase in the phyla Firmicutes/Bacteroidetes (F/B) ratio of the GID rats was restored by XEFP as well. Moreover, XEFP can relief the symptoms of GID rats by increasing bacteria Ruminococcaceae and decreasing Streptococcaceae, Erysipelotrichaceae and Lachnospiraceae in faecal microbiota level. This study represents a comprehensive survey of the interaction between GID and the intestinal flora and a systematic evaluation of modulation by a multicomponent drug.
关键词	XEFP functional dyspepsia intestinal flora metabonomics network pharmacology
DOI	10.3389/fphar.2021.670335
收录类别	SCI
语种	英语
资助项目	Strategic Scientific and Technological Project of Hunan Province[2016GK4050]
WOS研究方向	Pharmacology & Pharmacy
WOS类目	Pharmacology & Pharmacy
WOS记录号	WOS:000719825800001
出版者	FRONTIERS MEDIA SA
引用统计	被引频次：5[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://119.78.100.204/handle/2XEOYT63/18096
专题	中国科学院计算技术研究所期刊论文_英文
通讯作者	Wei, Junying; Zhao, Yi; Yang, Hongjun
作者单位	1.China Acad Chinese Med Sci, Inst Chinese Mat Med, Beijing, Peoples R China 2.Chinese Acad Tradit Chinese Med, Med Expt Ctr, Beijing, Peoples R China 3.Chinese Acad Sci, Inst Comp Technol, Adv Comp Res Ctr, Key Lab Intelligent Informat Proc, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Wu, Yue,Wu, Yang,Wu, Hongwei,et al. Systematic Survey of the Alteration of the Faecal Microbiota in Rats With Gastrointestinal Disorder and Modulation by Multicomponent Drugs[J]. FRONTIERS IN PHARMACOLOGY,2021,12:13.
APA	Wu, Yue.,Wu, Yang.,Wu, Hongwei.,Wu, Changxun.,Ji, Enhui.,...&Yang, Hongjun.(2021).Systematic Survey of the Alteration of the Faecal Microbiota in Rats With Gastrointestinal Disorder and Modulation by Multicomponent Drugs.FRONTIERS IN PHARMACOLOGY,12,13.
MLA	Wu, Yue,et al."Systematic Survey of the Alteration of the Faecal Microbiota in Rats With Gastrointestinal Disorder and Modulation by Multicomponent Drugs".FRONTIERS IN PHARMACOLOGY 12(2021):13.