Exploring the Microbiome-Wide Lysine Acetylation, Succinylation, and Propionylation in Human Gut Microbiota

doi:10.1021/acs.analchem.1c00962

	Exploring the Microbiome-Wide Lysine Acetylation, Succinylation, and Propionylation in Human Gut Microbiota
	Zhang, Xu 1,2; Cheng, Kai 1,2; Ning, Zhibin 1,2; Mayne, Janice 1,2; Walker, Krystal 1,2; Chi, Hao 3; Farnsworth, Charles L.4; Lee, Kimberly 4; Figeys, Daniel 1,2
	2021-05-04
发表期刊	ANALYTICAL CHEMISTRY
ISSN	0003-2700
卷号	93 期号:17 页码:6594-6598
摘要	Lysine acylations are important post-translational modifications that are present in both eukaryotes and prokaryotes and regulate diverse cellular functions. Our knowledge of the microbiome lysine acylation remains limited due to the lack of efficient analytical and bioinformatics methods for complex microbial communities. Here, we show that the serial enrichment using motif antibodies successfully captures peptides containing lysine acetylation, propionylation, and succinylation from human gut microbiome samples. A new bioinformatic workflow consisting of an unrestricted database search confidently identified >60,000 acetylated, and similar to 20,000 propionylated and succinylated gut microbial peptides. The characterization of these identified modification-specific metaproteomes, i.e., meta-PTMomes, demonstrates that lysine acylations are differentially distributed in microbial species with different metabolic capabilities. This study provides an analytical framework for the study of lysine acylations in the microbiome, which enables functional microbiome studies at the post-translational level.
DOI	10.1021/acs.analchem.1c00962
收录类别	SCI
语种	英语
资助项目	Government of Canada through Genome Canada ; Ontario Genomics Institute[OGI-156] ; Ontario Genomics Institute[OGI-149] ; Natural Sciences and Engineering Research Council of Canada (NSERC)[210034] ; Ontario Ministry of Economic Development and Innovation[ORF-DIG-14405] ; Ontario Ministry of Economic Development and Innovation[13440]
WOS研究方向	Chemistry
WOS类目	Chemistry, Analytical
WOS记录号	WOS:000648505900004
出版者	AMER CHEMICAL SOC
引用统计	被引频次：11[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://119.78.100.204/handle/2XEOYT63/17690
专题	中国科学院计算技术研究所期刊论文_英文
通讯作者	Figeys, Daniel
作者单位	1.Univ Ottawa, Fac Med, Ottawa Inst Syst Biol, Ottawa, ON K1H 8M5, Canada 2.Univ Ottawa, Fac Med, Dept Biochem Microbiol & Immunol, Ottawa, ON K1H 8M5, Canada 3.Chinese Acad Sci, Inst Comp Technol, Beijing 100190, Peoples R China 4.Cell Signaling Technol Inc, Danvers, MA 01923 USA
推荐引用方式 GB/T 7714	Zhang, Xu,Cheng, Kai,Ning, Zhibin,et al. Exploring the Microbiome-Wide Lysine Acetylation, Succinylation, and Propionylation in Human Gut Microbiota[J]. ANALYTICAL CHEMISTRY,2021,93(17):6594-6598.
APA	Zhang, Xu.,Cheng, Kai.,Ning, Zhibin.,Mayne, Janice.,Walker, Krystal.,...&Figeys, Daniel.(2021).Exploring the Microbiome-Wide Lysine Acetylation, Succinylation, and Propionylation in Human Gut Microbiota.ANALYTICAL CHEMISTRY,93(17),6594-6598.
MLA	Zhang, Xu,et al."Exploring the Microbiome-Wide Lysine Acetylation, Succinylation, and Propionylation in Human Gut Microbiota".ANALYTICAL CHEMISTRY 93.17(2021):6594-6598.