Transcriptome landscape of double negative T cells by single-cell RNA sequencing

doi:10.1016/j.jaut.2021.102653

	Transcriptome landscape of double negative T cells by single-cell RNA sequencing
	Yang, Lu 1,2,3; Zhu, Yanbing 3,4,5; Tian, Dan 3,4,5; Wang, Song 3,4,5; Guo, Jincheng 6; Sun, Guangyong 3,4,5; Jin, Hua 3,4,5; Zhang, Chunpan 3,4,5; Shi, Wen 3,4,5; Gershwin, M. Eric 7; Zhang, Zhongtao 1,2; Zhao, Yi 6; Zhang, Dong 1,2,3,4,5
	2021-07-01
发表期刊	JOURNAL OF AUTOIMMUNITY
ISSN	0896-8411
卷号	121 页码:15
摘要	CD4 and CD8 coreceptor double negative TCR alpha beta+ T (DNT) cells are increasingly being recognized for their critical and diverse roles in the immune system. However, their molecular and functional signatures remain poorly understood and controversial. Moreover, the majority of studies are descriptive because of the relative low frequency of cells and non-standardized definition of this lineage. In this study, we performed single-cell RNA sequencing on 28,835 single immune cells isolated from mixed splenocytes of male C57BL/6 mice using strict fluorescence-activated cell sorting. The data was replicated in a subsequent study. Our analysis revealed five transcriptionally distinct nave DNT cell clusters, which expressed unique sets of genes and primarily performed T helper, cytotoxic and innate immune functions. Anti-CD3/CD28 activation enhanced their T helper and cytotoxic functions. Moreover, in comparison with CD4+, CD8+ T cells and NK cells, Ikzf2 was highly expressed by both nave and activated cytotoxic DNT cells. In conclusion, we provide a map of the heterogeneity in nave and active DNT cells, addresses the controversy about DNT cells, and provides potential transcription signatures of DNT cells. The landscape approach herein will eventually become more feasible through newer high throughput methods and will enable clustering data to be fed into a systems analysis approach. Thus the approach should become the "backdrop" of similar studies in the myriad murine models of autoimmunity, potentially highlighting the importance of DNT cells and other minor lineage of cells in immune homeostasis. The clear characterization of functional DNT subsets into helper DNT, cytotoxic DNT and innate DNT will help to better understand the intrinsic roles of different functional DNT subsets in the development and progression of autoimmune diseases and transplant rejection, and thereby may facilitate diagnosis and therapy.
关键词	Double negative T cell Single-cell RNA sequencing Unconventional T cells Inflammation Regulatory T cells Innate immunity
DOI	10.1016/j.jaut.2021.102653
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[81570510] ; National Natural Science Foundation of China[81870399] ; National Natural Science Foundation of China[82001694]
WOS研究方向	Immunology
WOS类目	Immunology
WOS记录号	WOS:000663804000001
出版者	ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
引用统计	被引频次：26[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://119.78.100.204/handle/2XEOYT63/17639
专题	中国科学院计算技术研究所期刊论文_英文
通讯作者	Gershwin, M. Eric; Zhang, Zhongtao; Zhao, Yi; Zhang, Dong
作者单位	1.Capital Med Univ, Beijing Friendship Hosp, Gen Surg Dept, Beijing 100050, Peoples R China 2.Natl Clin Res Ctr Digest Dis, Beijing 100050, Peoples R China 3.Beijing Key Lab Tolerance Induct & Organ Protect, Beijing 100050, Peoples R China 4.Capital Med Univ, Beijing Friendship Hosp, Immunol Res Ctr Oral & Syst Hlth, Beijing, Peoples R China 5.Beijing Clin Res Inst, Beijing 100050, Peoples R China 6.Chinese Acad Sci, Adv Comp Res Ctr, Inst Comp Technol, Key Lab Intelligent Informat Proc, Beijing 100190, Peoples R China 7.Univ Calif Davis, Div Rheumatol Allergy & Clin Immunol, Davis, CA 95616 USA
推荐引用方式 GB/T 7714	Yang, Lu,Zhu, Yanbing,Tian, Dan,et al. Transcriptome landscape of double negative T cells by single-cell RNA sequencing[J]. JOURNAL OF AUTOIMMUNITY,2021,121:15.
APA	Yang, Lu.,Zhu, Yanbing.,Tian, Dan.,Wang, Song.,Guo, Jincheng.,...&Zhang, Dong.(2021).Transcriptome landscape of double negative T cells by single-cell RNA sequencing.JOURNAL OF AUTOIMMUNITY,121,15.
MLA	Yang, Lu,et al."Transcriptome landscape of double negative T cells by single-cell RNA sequencing".JOURNAL OF AUTOIMMUNITY 121(2021):15.