Layers of interstitial fluid flow along a "slit-shaped" vascular adventitia

doi:10.1631/jzus.B2000590

	Layers of interstitial fluid flow along a "slit-shaped" vascular adventitia
	Li, Hongyi 1,2; Lyu, You 2; Chen, Xiaoliang 3; Li, Bei 2; Hua, Qi 1; Ji, Fusui 2; Yin, Yajun 4; Li, Hua 5
	2021-07-01
发表期刊	JOURNAL OF ZHEJIANG UNIVERSITY-SCIENCE B
ISSN	1673-1581
卷号	22 期号:8 页码:647-663
摘要	Interstitial fluid (ISF) flow through vascular adventitia has been discovered recently. However, its kinetic pattern was unclear. We used histological and topographical identification to observe ISF flow along venous vessels in rabbits. By magnetic resonance imaging (MRI) in live subjects, the inherent pathways of ISF flow from the ankle dermis through the legs, abdomen, and thorax were enhanced by paramagnetic contrast. By fluorescence stereomicroscopy and layer-by-layer dissection after the rabbits were sacrificed, the perivascular and adventitial connective tissues (PACTs) along the saphenous veins and inferior vena cava were found to be stained by sodium fluorescein from the ankle dermis, which coincided with the findings by MRI. The direction of ISF transport in a venous PACT pathway was the same as that of venous blood flow. By confocal microscopy and histological analysis, the stained PACT pathways were verified to be the fibrous connective tissues, consisting of longitudinally assembled fibers. Real-time observations by fluorescence stereomicroscopy revealed at least two types of spaces for ISF flow: one along adventitial fibers and another one between the vascular adventitia and its covering fascia. Using nanoparticles and surfactants, a PACT pathway was found to be accessible by a nanoparticle of <100 nm and contained two parts: a transport channel and an absorptive part. The calculated velocity of continuous ISF flow along fibers of the PACT pathway was 3.6-15.6 mm/s. These data revealed that a PACT pathway was a "slit-shaped" porous biomaterial, comprising a longitudinal transport channel and an absorptive part for imbibition. The use of surfactants suggested that interfacial tension might play an essential role in layers of continuous ISF flow along vascular vessels. A hypothetical "gel pump" is proposed based on interfacial tension and interactions to regulate ISF flow. These experimental findings may inspire future studies to explore the physiological and pathophysiological functions of vascular ISF or interfacial fluid flow among interstitial connective tissues throughout the body.
关键词	Vascular adventitia Interstitial fluid Connective tissue Interfacial zone
DOI	10.1631/jzus.B2000590
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[82050004] ; National Natural Science Foundation of China[81141118] ; Beijing Hospital Clinical Research 121 Project[121-2016002] ; National Basic Research Program of China[2015CB554507] ; Weiwu HU
WOS研究方向	Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Research & Experimental Medicine
WOS类目	Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Medicine, Research & Experimental
WOS记录号	WOS:000690909200003
出版者	ZHEJIANG UNIV
引用统计	被引频次：3[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://119.78.100.204/handle/2XEOYT63/17107
专题	中国科学院计算技术研究所期刊论文_英文
通讯作者	Li, Hongyi; Hua, Qi
作者单位	1.Capital Med Univ, Xuanwu Hosp, Cardiol Dept, Beijing 100053, Peoples R China 2.Chinese Acad Med Sci, Inst Geriatr Med, Natl Ctr Gerontol, Cardiol Dept,Beijing Hosp, Beijing 100730, Peoples R China 3.China Japan Friendship Hosp, Radiol Dept, Beijing 100029, Peoples R China 4.Tsinghua Univ, Dept Engn Mech, Beijing 100084, Peoples R China 5.Chinese Acad Sci, Inst Comp Technol, Beijing 100190, Peoples R China
推荐引用方式 GB/T 7714	Li, Hongyi,Lyu, You,Chen, Xiaoliang,et al. Layers of interstitial fluid flow along a "slit-shaped" vascular adventitia[J]. JOURNAL OF ZHEJIANG UNIVERSITY-SCIENCE B,2021,22(8):647-663.
APA	Li, Hongyi.,Lyu, You.,Chen, Xiaoliang.,Li, Bei.,Hua, Qi.,...&Li, Hua.(2021).Layers of interstitial fluid flow along a "slit-shaped" vascular adventitia.JOURNAL OF ZHEJIANG UNIVERSITY-SCIENCE B,22(8),647-663.
MLA	Li, Hongyi,et al."Layers of interstitial fluid flow along a "slit-shaped" vascular adventitia".JOURNAL OF ZHEJIANG UNIVERSITY-SCIENCE B 22.8(2021):647-663.