Transcriptomic analysis of glioblastoma multiforme providing new insights into GPR17 signaling communication

doi:10.1080/07391102.2020.1841029

	Transcriptomic analysis of glioblastoma multiforme providing new insights into GPR17 signaling communication
	Mutharasu, Gnanavel 1,2; Murugesan, Akshaya 1,2,3,4; Konda Mani, Saravanan 5; Yli-Harja, Olli 1,2,6,7; Kandhavelu, Meenakshisundaram 1,2,3,8
	2020-10-30
发表期刊	JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
ISSN	0739-1102
页码	14
摘要	Glioblastoma Multiforme (GBM) is one of the most aggressive malignant tumors in the central nervous system, which arises due to the failure or crosstalk in the signaling networks. GPR17, an orphan G protein-coupled receptor is anticipated to be associated with the biology of the GBM disease progression. In the present study, we have identified the differential expressions of around 170 genes along with GPR17 through the RNA-Seq analysis of 169 GBM samples. Coordinated expression patterns of all other gene products with this receptor were analysed using gene ontology and protein-protein interaction data. Several crucial signaling components and genes that play a significant role in tumor progression have been identified among which GPR17 was found to be significantly interacting with about 30 different pathways. High-throughput molecular docking of GPR17 by homology-based model against differentially expressed proteins, showed effective recognition and binding of PX, SH3, and Ig-like domains besides Gi protein. Pathways of PI3, Src, Ptdn, Ras, cytoplasmic tyrosine kinases, phospholipases, nexins and other proteins possessing these structural domains are identified as critical signaling components of the complex GBM signaling network. Our findings also provide a mechanistic insight of GPR17-T0510-3657 interaction, which potentially regulates the interaction of PX domain and helical mPTS recognition domain-containing proteins. Overall, our results demonstrate that GPR17 mediated signaling networks could be used as a therapeutic target for GBM. Communicated by Ramaswamy H. Sarma
关键词	GPR17 signaling networks glioblastoma multiforme RNA-Seq protein– protein interface kinases
DOI	10.1080/07391102.2020.1841029
收录类别	SCI
语种	英语
资助项目	Center for International Mobility (CIMO) Finland[TM-13-8898] ; Center for International Mobility (CIMO) Finland[KM-14-9107] ; Center for International Mobility (CIMO) Finland[TM-15-9720] ; Finnish cultural foundation[0116947-3]
WOS研究方向	Biochemistry & Molecular Biology ; Biophysics
WOS类目	Biochemistry & Molecular Biology ; Biophysics
WOS记录号	WOS:000584061400001
出版者	TAYLOR & FRANCIS INC
引用统计	被引频次：13[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://119.78.100.204/handle/2XEOYT63/15482
专题	中国科学院计算技术研究所期刊论文_英文
通讯作者	Kandhavelu, Meenakshisundaram
作者单位	1.Tampere Univ, BioMediTech Inst, Tampere, Finland 2.Tampere Univ, Fac Med & Hlth Technol, Tampere, Finland 3.Tampere Univ, Mol Signalling Lab, Fac Med & Hlth Technol, Tampere, Finland 4.Lady Doak Coll, Dept Biotechnol, Madurai, Tamil Nadu, India 5.Chinese Acad Sci, Shenzhen Inst Adv Technol, Ctr High Performance Comp, Shenzhen, Peoples R China 6.Tampere Univ, Fac Med & Hlth Technol, Computa Syst Biol Grp, Tampere, Finland 7.Inst Syst Biol, Seattle, WA USA 8.Tampere Univ Hosp, Sci Ctr, Tampere, Finland
推荐引用方式 GB/T 7714	Mutharasu, Gnanavel,Murugesan, Akshaya,Konda Mani, Saravanan,et al. Transcriptomic analysis of glioblastoma multiforme providing new insights into GPR17 signaling communication[J]. JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS,2020:14.
APA	Mutharasu, Gnanavel,Murugesan, Akshaya,Konda Mani, Saravanan,Yli-Harja, Olli,&Kandhavelu, Meenakshisundaram.(2020).Transcriptomic analysis of glioblastoma multiforme providing new insights into GPR17 signaling communication.JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS,14.
MLA	Mutharasu, Gnanavel,et al."Transcriptomic analysis of glioblastoma multiforme providing new insights into GPR17 signaling communication".JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS (2020):14.