Identification of carbohydrate peripheral epitopes important for recognition by positive-ion MALDI multistage mass spectrometry

doi:10.1016/j.carbpol.2019.115528

	Identification of carbohydrate peripheral epitopes important for recognition by positive-ion MALDI multistage mass spectrometry
	Huang, Chuncui 1; Sun, Shiwei 2,3; Yan, Jingyu 4; Wang, Hui 2; Zhou, Jinyu 1; Gao, Huanyu 1; Xie, Wenchun 1; Li, Yan 1,3; Chai, Wengang 5
	2020-02-01
发表期刊	CARBOHYDRATE POLYMERS
ISSN	0144-8617
卷号	229 页码:13
摘要	Carbohydrate sequences are important for various biological processes. It has recently been estimated to have 100,000-500,000 carbohydrate structures in mammalian glycome. However, the peripheral carbohydrate determinants on N- and O-glycoproteins, glycolipids, polysaccharides and secreted free sugars are limited in numbers. Among these blood-group-related antigens the ABO(H)- and Lewis-types are particularly important. Negative-ion MS/MS has been successfully used in assignment of these epitopes on free reducing sugars but cannot be applied to reduced sugars, e.g. O-glycans typically released from mucins as alditols, or in positive-ion detection of either reducing or reduced oligosaccharides. In the present study, we investigate the fragmentation features of permethylated reducing and reduced sugars under positive-ion conditions of multi-stage MALDI-MS, and propose the concept of epitope ion and epitope spectrum for determination of peripheral blood-group related epitopes on secreted human milk oligosaccharides and N-glycans as reducing sugars and O-glycans as reduced alditols in conjunction with MALDI-MS glycan profiling.
关键词	Oligosaccharide Carbohydrate epitope Blood-group antigen Lewis antigen MALDI-MS
DOI	10.1016/j.carbpol.2019.115528
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[31600650] ; National Natural Science Foundation of China[31671369] ; National Natural Science Foundation of China[31770775] ; March of Dimes Prematurity Research Center[22-FY18-821]
WOS研究方向	Chemistry ; Polymer Science
WOS类目	Chemistry, Applied ; Chemistry, Organic ; Polymer Science
WOS记录号	WOS:000501796600061
出版者	ELSEVIER SCI LTD
引用统计	被引频次：8[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://119.78.100.204/handle/2XEOYT63/14991
专题	中国科学院计算技术研究所期刊论文_英文
通讯作者	Li, Yan; Chai, Wengang
作者单位	1.Chinese Acad Sci, Inst Biophys, 15 Datun Rd, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Inst Comp Technol, Key Lab Intelligent Informat Proc, 6 Kexueyuan South Rd, Beijing 100080, Peoples R China 3.Univ Chinese Acad Sci, 19 Yuquan Rd, Beijing 100049, Peoples R China 4.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China 5.Imperial Coll London, Glycosci Lab, Fac Med, Hammersmith Campus, London W12 0NN, England
推荐引用方式 GB/T 7714	Huang, Chuncui,Sun, Shiwei,Yan, Jingyu,et al. Identification of carbohydrate peripheral epitopes important for recognition by positive-ion MALDI multistage mass spectrometry[J]. CARBOHYDRATE POLYMERS,2020,229:13.
APA	Huang, Chuncui.,Sun, Shiwei.,Yan, Jingyu.,Wang, Hui.,Zhou, Jinyu.,...&Chai, Wengang.(2020).Identification of carbohydrate peripheral epitopes important for recognition by positive-ion MALDI multistage mass spectrometry.CARBOHYDRATE POLYMERS,229,13.
MLA	Huang, Chuncui,et al."Identification of carbohydrate peripheral epitopes important for recognition by positive-ion MALDI multistage mass spectrometry".CARBOHYDRATE POLYMERS 229(2020):13.