Long-term, in toto live imaging of cardiomyocyte behaviour during mouse ventricle chamber formation at single-cell resolution

doi:10.1038/s41556-020-0475-2

	Long-term, in toto live imaging of cardiomyocyte behaviour during mouse ventricle chamber formation at single-cell resolution
	Yue, Yanzhu 1; Zong, Weijian 1; Li, Xin 1; Li, Jinghang 1; Zhang, Youdong 1; Wu, Runlong 1,2; Liu, Yazui 3; Cui, Jiahao 3; Wang, Qianhao 1; Bian, Yunkun 1; Yu, Xianhong 1; Liu, Yao 4; Tan, Guangming 4; Zhang, Yunfeng 2; Zhao, Gang 3; Zhou, Bin 5; Chen, Liangyi 1; Xiao, Wenlei 3; Cheng, Heping 1; He, Aibin 1
	2020-03-01
发表期刊	NATURE CELL BIOLOGY
ISSN	1465-7392
卷号	22 期号:3 页码:332-+
摘要	Yue, Zong, Li, Li, Zhang, Wu et al. introduce an in toto live-imaging system to track cardiac ventricle chamber formation at single-cell resolution for up to 1.5 days and digitally reconstruct cell dynamics. Mapping of the holistic cell behaviours sculpting the four-chambered mammalian heart has been a goal or previous studies, but so far only success in transparent invertebrates and lower vertebrates with two-chambered hearts has been achieved. Using a live-imaging system comprising a customized vertical light-sheet microscope equipped with a mouse embryo culture module, a heartbeat-gated imaging strategy and a digital image processing framework, we realized volumetric imaging of developing mouse hearts at single-cell resolution and with uninterrupted cell lineages for up to 1.5 d. Four-dimensional landscapes of Nppa(+) cardiomyocyte cell behaviours revealed a blueprint for ventricle chamber formation by which biased outward migration of the outermost cardiomyocytes is coupled with cell intercalation and horizontal division. The inner-muscle architecture of trabeculae was developed through dual mechanisms: early fate segregation and transmural cell arrangement involving both oriented cell division and directional migration. Thus, live-imaging reconstruction of uninterrupted cell lineages affords a transformative means for deciphering mammalian organogenesis.
DOI	10.1038/s41556-020-0475-2
收录类别	SCI
语种	英语
资助项目	National Basic Research Program of China[2017YFA0103402] ; National Basic Research Program of China[2019YFA0801802] ; National Basic Research Program of China[2016YFA0500403] ; National Natural Science Foundation of China[31571487] ; National Natural Science Foundation of China[31771607] ; National Natural Science Foundation of China[31327901] ; National Natural Science Foundation of China[31521062] ; Peking-Tsinghua Center for Life Sciences ; 1000 Youth Talents Program of China ; National Key Technologies RD Program[SQ2011SF11B01041] ; National Key Technologies RD Program[2018YFA0109600] ; National Postdoctoral Program for Innovative Talents[8206200030]
WOS研究方向	Cell Biology
WOS类目	Cell Biology
WOS记录号	WOS:000518502700011
出版者	NATURE PUBLISHING GROUP
引用统计	被引频次：2[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://119.78.100.204/handle/2XEOYT63/14472
专题	中国科学院计算技术研究所期刊论文_英文
通讯作者	Xiao, Wenlei; Cheng, Heping; He, Aibin
作者单位	1.Peking Univ, Peking Tsinghua Ctr Life Sci, Beijing Key Lab Cardiometab Mol Med, State Key Lab Membrane Biol,Inst Mol Med, Beijing, Peoples R China 2.Peking Univ, Sch Elect Engn & Comp Sci, State Key Lab Adv Opt Commun Syst & Networks, Beijing, Peoples R China 3.Beihang Univ, Sch Mech Engn & Automat, Beijing, Peoples R China 4.Chinese Acad Sci, Inst Comp Technol, Beijing, Peoples R China 5.Univ Chinese Acad Sci, Inst Biochem & Cell Biol, CAS Ctr Excellence Mol Cell Sci, State Key Lab Cell Biol, Shanghai, Peoples R China
推荐引用方式 GB/T 7714	Yue, Yanzhu,Zong, Weijian,Li, Xin,et al. Long-term, in toto live imaging of cardiomyocyte behaviour during mouse ventricle chamber formation at single-cell resolution[J]. NATURE CELL BIOLOGY,2020,22(3):332-+.
APA	Yue, Yanzhu.,Zong, Weijian.,Li, Xin.,Li, Jinghang.,Zhang, Youdong.,...&He, Aibin.(2020).Long-term, in toto live imaging of cardiomyocyte behaviour during mouse ventricle chamber formation at single-cell resolution.NATURE CELL BIOLOGY,22(3),332-+.
MLA	Yue, Yanzhu,et al."Long-term, in toto live imaging of cardiomyocyte behaviour during mouse ventricle chamber formation at single-cell resolution".NATURE CELL BIOLOGY 22.3(2020):332-+.