Fast Super-Resolution Imaging Technique and Immediate Early Nanostructure Capturing by a Photoconvertible Fluorescent Protein

doi:10.1021/acs.nanolett.9b02855

	Fast Super-Resolution Imaging Technique and Immediate Early Nanostructure Capturing by a Photoconvertible Fluorescent Protein
	Zhang, Mingshu 1; Fu, Zhifei 1,2; Li, Changqing 1; Liu, Anyuan 1,3; Peng, Dingming 1,2; Xue, Fudong 1,2; He, Wenting 1; Gao, Shan 2,4; Xu, Fan 4; Xu, Dan 5; Yuan, Ling 6; Zhang, Fa 4; Xu, Zhiheng 2,7; Xu, Tao 2,8; Xu, Pingyong 1,2,8
	2020-04-08
发表期刊	NANO LETTERS
ISSN	1530-6984
卷号	20 期号:4 页码:2197-2208
摘要	Low temporal resolution and limited photo-controllable fluorescent protein probes have restricted the widespread application of single-molecule localization microscopy (SMLM). In the current study, we developed a new photo-convertible fluorescent protein (PCFP), pcStar, and quick single molecule-guided Bayesian localization microscopy (Quick-SIMBA). The combination of pcStar and Quick-SIMBA achieved the highest temporal resolution (0.1-0.25 s) with large field-of-view (76 X 9.4 mu m(2) -76 X 31.4 mu m(2)) among the SMLM methods, which enabled the dynamic movements of the endoplasmic reticulum dense tubular matrix to be resolved. Moreover, pcStar extended the application of SMLM to imaging the immediate early nanostructures in Drosophila embryos and revealed a specific "parallel three-pillar" structure in the neuronal-glial cell junction, helping to elucidate glial cell "locking" and support of neurons during Drosophila embryogenesis.
关键词	Photoconvertible fluorescent protein live super-resolution microscopy Drosophila embryos Quick-SIMBA pcStar
DOI	10.1021/acs.nanolett.9b02855
收录类别	SCI
语种	英语
资助项目	National Key R&D Program of China[2017YFA0505300] ; National Key R&D Program of China[2016YFA0501500] ; National Natural Science Foundation of China[31670870] ; National Natural Science Foundation of China[31870857] ; National Natural Science Foundation of China[31421002] ; National Natural Science Foundation of China[21778069] ; Chinese Academy of Sciences[XDB08030203] ; Project of Chinese Academy of Sciences-Peking University Leading Cooperation Team
WOS研究方向	Chemistry ; Science & Technology - Other Topics ; Materials Science ; Physics
WOS类目	Chemistry, Multidisciplinary ; Chemistry, Physical ; Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary ; Physics, Applied ; Physics, Condensed Matter
WOS记录号	WOS:000526413400001
出版者	AMER CHEMICAL SOC
引用统计	被引频次：18[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://119.78.100.204/handle/2XEOYT63/14200
专题	中国科学院计算技术研究所期刊论文_英文
通讯作者	Xu, Tao; Xu, Pingyong
作者单位	1.Chinese Acad Sci, Inst Biophys, Key Lab RNA Biol, Beijing 100101, Peoples R China 2.Univ Chinese Acad Sci, Coll Life Sci, Beijing 100149, Peoples R China 3.Univ Sci & Technol China, Sch Life Sci, Hefei 230026, Anhui, Peoples R China 4.Chinese Acad Sci, Inst Comp Technol, Key Lab Intelligent Informat Proc, Beijing 100190, Peoples R China 5.Fuzhou Univ, Coll Biol Sci & Engn, Inst Life Sci, Fuzhou 350116, Peoples R China 6.Cent South Univ, Ctr Med Genet, Sch Life Sci, Changsha 410078, Hunan, Peoples R China 7.Chinese Acad Sci, CAS Ctr Excellence Brain Sci & Intelligence Techn, Inst Genet & Dev Biol, State Key Lab Mol Dev Biol, Beijing 100101, Peoples R China 8.Chinese Acad Sci, CAS Ctr Excellence Biomacromol, Inst Biophys, Natl Lab Biomacromol, Beijing 100101, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Mingshu,Fu, Zhifei,Li, Changqing,et al. Fast Super-Resolution Imaging Technique and Immediate Early Nanostructure Capturing by a Photoconvertible Fluorescent Protein[J]. NANO LETTERS,2020,20(4):2197-2208.
APA	Zhang, Mingshu.,Fu, Zhifei.,Li, Changqing.,Liu, Anyuan.,Peng, Dingming.,...&Xu, Pingyong.(2020).Fast Super-Resolution Imaging Technique and Immediate Early Nanostructure Capturing by a Photoconvertible Fluorescent Protein.NANO LETTERS,20(4),2197-2208.
MLA	Zhang, Mingshu,et al."Fast Super-Resolution Imaging Technique and Immediate Early Nanostructure Capturing by a Photoconvertible Fluorescent Protein".NANO LETTERS 20.4(2020):2197-2208.