Single-cell RNA sequencing of t(8;21) acute myeloid leukemia for risk prediction

doi:10.3892/or.2020.7507

	Single-cell RNA sequencing of t(8;21) acute myeloid leukemia for risk prediction
	Xiong, Qian 1,2,3; Huang, Sai 1,2; Li, Yong-Hui 1,2; Lv, Na 1,2,4; Lv, Chao 1,2; Ding, Yi 1,2; Liu, Wen-Wen 1,2; Wang, Li-Li 1,2; Chen, Yang 5,6; Sun, Liang 5,6; Zhao, Yi 7; Liao, Sheng-You 7; Zhang, Michael Q.5,6; Zhu, Bao-Li 3,8,9,10; Yu, Li 1,2,4
	2020-04-01
发表期刊	ONCOLOGY REPORTS
ISSN	1021-335X
卷号	43 期号:4 页码:1278-1288
摘要	Single-cell RNA sequencing (scRNA-seq) of bone marrow or peripheral blood samples from patients with acute myeloid leukemia (AML) enables the characterization of heterogeneous malignant cells. A total of 87 cells from two patients with t(8;21) AML were analyzed using scRNA-seq. Clustering methods were used to separate leukemia cells into different sub-populations, and the expression patterns of specific marker genes were used to annotate these populations. Among the 31 differentially expressed genes in the cells of a patient who relapsed after hematopoietic stem cell transplantation, 13 genes were identified to be associated with leukemia. Furthermore, three genes, namely AT-rich interaction domain 2, lysine methyltransferase 2A and synaptotagmin binding cytoplasmic RNA interacting protein were validated as possible prognostic biomarkers using two bulk expression datasets. Taking advantage of scRNA-seq, the results of the present study may provide clinicians with several possible biomarkers to predict the prognostic outcomes of t(8;21) AML.
关键词	acute myeloid leukemia t(8 21) single-cell RNA-sequencing prognosis
DOI	10.3892/or.2020.7507
收录类别	SCI
语种	英语
资助项目	National Natural Science Fund[81670162] ; PLA General Hospital Science and Technology Project[18KMM01] ; Beijing Natural Science Foundation[7204305]
WOS研究方向	Oncology
WOS类目	Oncology
WOS记录号	WOS:000523753100022
出版者	SPANDIDOS PUBL LTD
引用统计	被引频次：4[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://119.78.100.204/handle/2XEOYT63/14119
专题	中国科学院计算技术研究所期刊论文_英文
通讯作者	Zhu, Bao-Li; Yu, Li
作者单位	1.Chinese Peoples Liberat Army Gen Hosp, Dept Hematol, 28 Fuxing Rd, Beijing 100853, Peoples R China 2.Chinese Peoples Liberat Army Gen Hosp, BMT Ctr, 28 Fuxing Rd, Beijing 100853, Peoples R China 3.Chinese Acad Sci, Inst Microbiol, CAS Key Lab Pathogen Microbiol & Immunol, 1 Beichen West Rd, Beijing 100101, Peoples R China 4.Shenzhen Univ, Hlth Sci Ctr, Gen Hosp, Dept Hematol Oncol,Int Canc Ctr, Shenzhen 518060, Guangdong, Peoples R China 5.Tsinghua Univ, Sch Med, MOE Key Lab Bioinformat, Beijing 100084, Peoples R China 6.Tsinghua Univ, Bioinformat Div, Ctr Synthet & Syst Biol, TNLIST,Dept Automat, Beijing 100084, Peoples R China 7.Chinese Acad Sci, Inst Comp Technol, Adv Comp Res Ctr, Key Lab Intelligent Informat Proc, Beijing 100190, Peoples R China 8.Univ Chinese Acad Sci, Savaid Med Sch, Beijing 100049, Peoples R China 9.Beijing Key Lab Antimicrobial Resistance & Pathog, Beijing 100101, Peoples R China 10.Southwest Med Univ, Sch Basic Med Sci, Dept Pathogen Biol, Luzhou 646000, Sichuan, Peoples R China
推荐引用方式 GB/T 7714	Xiong, Qian,Huang, Sai,Li, Yong-Hui,et al. Single-cell RNA sequencing of t(8;21) acute myeloid leukemia for risk prediction[J]. ONCOLOGY REPORTS,2020,43(4):1278-1288.
APA	Xiong, Qian.,Huang, Sai.,Li, Yong-Hui.,Lv, Na.,Lv, Chao.,...&Yu, Li.(2020).Single-cell RNA sequencing of t(8;21) acute myeloid leukemia for risk prediction.ONCOLOGY REPORTS,43(4),1278-1288.
MLA	Xiong, Qian,et al."Single-cell RNA sequencing of t(8;21) acute myeloid leukemia for risk prediction".ONCOLOGY REPORTS 43.4(2020):1278-1288.