Multistage mass spectrometry with intelligent precursor selection for N-glycan branching pattern analysis

doi:10.1016/j.carbpol.2020.116122

	Multistage mass spectrometry with intelligent precursor selection for N-glycan branching pattern analysis
	Huang, Chuncui 1; Wang, Hui 2,3; Bu, Dongbo 2,3; Zhou, Jinyu 1; Dong, Junchuan 2,3; Zhang, Jingwei 2,3; Gao, Huanyu 1; Wang, Yaojun 2; Chai, Wengang 4; Sun, Shiwei 2,3; Li, Yan 1,3
	2020-06-01
发表期刊	CARBOHYDRATE POLYMERS
ISSN	0144-8617
卷号	237 页码:12
摘要	Biological functions of N-glycans are frequently related to their unique branching patterns. Multistage mass spectrometry (MSn) has become the primary method for glycan structural analysis. However, selection of the best fragment as the precursor for the next round of product-ion scanning is important but difficult. We have previously proposed the concept and designed the approach of glycan intelligent precursor selection (GIPS) to guide MSn experiments, but its use in N-glycans is not straightforward as some N-glycans are of high similarity in branching patterns. In the present work we introduced new elements to GIPS to improve its performance in N-glycan branching pattern analysis. These include a hypothesis and significance test, based on Bayes factor, and DPbiased as a new precursor selection strategy. The improved GIPS was successfully applied to identification of individual N-glycans, and incorporated into MALDI MS N-glycan profiling for assignment of N-glycans obtained from glycoproteins and complex human serum.
关键词	Intelligent precursor selection N-gGlycan branching pattern Multistage mass spectrometry Distinctive fragments Hypothesis and significance test
DOI	10.1016/j.carbpol.2020.116122
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[31600650] ; National Natural Science Foundation of China[31671369] ; National Natural Science Foundation of China[31770775] ; March of Dimes Prematurity Research Center grant[22-FY18-821] ; Wellcome Trust Biomedical Resource grant[WT 218304/Z/19/Z]
WOS研究方向	Chemistry ; Polymer Science
WOS类目	Chemistry, Applied ; Chemistry, Organic ; Polymer Science
WOS记录号	WOS:000522387000030
出版者	ELSEVIER SCI LTD
引用统计	被引频次：7[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://119.78.100.204/handle/2XEOYT63/14107
专题	中国科学院计算技术研究所期刊论文_英文
通讯作者	Chai, Wengang; Sun, Shiwei; Li, Yan
作者单位	1.Chinese Acad Sci, Inst Biophys, 15 Datun Rd, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Inst Comp Technol, Key Lab Intelligent Informat Proc, 6 Kexueyuan South Rd, Beijing 100080, Peoples R China 3.Univ Chinese Acad Sci, 19 Yuquan Rd, Beijing 100049, Peoples R China 4.Imperial Coll London, Fac Med, Glycosci Lab, London W12 0NN, England
推荐引用方式 GB/T 7714	Huang, Chuncui,Wang, Hui,Bu, Dongbo,et al. Multistage mass spectrometry with intelligent precursor selection for N-glycan branching pattern analysis[J]. CARBOHYDRATE POLYMERS,2020,237:12.
APA	Huang, Chuncui.,Wang, Hui.,Bu, Dongbo.,Zhou, Jinyu.,Dong, Junchuan.,...&Li, Yan.(2020).Multistage mass spectrometry with intelligent precursor selection for N-glycan branching pattern analysis.CARBOHYDRATE POLYMERS,237,12.
MLA	Huang, Chuncui,et al."Multistage mass spectrometry with intelligent precursor selection for N-glycan branching pattern analysis".CARBOHYDRATE POLYMERS 237(2020):12.