Simultaneous Structural Identification of Constituents in Active Herbal Extract of Forsythia Suspensa Using Nuclear Magnetic Resonance/Liquid Chromatography-Mass Spectrometry Parallel Dynamic Spectroscopy

doi:10.3724/SP.J.1096.2011.00323

	Simultaneous Structural Identification of Constituents in Active Herbal Extract of Forsythia Suspensa Using Nuclear Magnetic Resonance/Liquid Chromatography-Mass Spectrometry Parallel Dynamic Spectroscopy
	Liu Ying 1,2; He Jiu-Ming 1,2; Sun Rui-Xiang 3; Liu Chao 3; Zhang Rui-Ping 1,2; Shi Jian-Gong 1,2; Zeper, Abliz 1,2
	2011-03-01
发表期刊	CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
ISSN	0253-3820
卷号	39 期号:3 页码:323-329
摘要	Based on an incomplete separation strategy, the crude extract of Forsythia Suspensa was separated into a series of fractions with the concentration of constituent dynamic variation by RP preparative chromatography. NMR/LC-MS parallel dynamic spectroscopic (PDS) method was applied to identify the phenylethanoid glycosides in these fractions. The complementary spectral information of the same individual constituent in the crude extract was simultaneously obtained from the spectra of mixtures, including retention time (R(t)), mass/charge (m/z) and chemical shift (delta). Seven constituents were identified successfully, including a new phenylethanoid glycoside and a novel Dimeric-2-(4-aminophenyl) ethyl-beta-D-glucopyranoside which is the first example of dimeric phenylethanol alkaloid glycoside. The results demonstrated that NMR/LC-MS PDS can be used to identify the constituents with more complicated structures from herbs and their active extracts. Especially, this method is suitable for analysis and identification of minimum-quantity constituents and constituents with similar polarity which are hardly obtained in routine experimental conditions. In addition, this method has the advantage of rapid discovering and identifying new compounds from complex mixtures, which can greatly improve the analytical efficiency.
关键词	Nuclear magnetic resonance/liquid chromatography/mass spectrometry parallel dynamic spectroscopy Incomplete separation strategy Simultaneous structural identification in mixture Forsythia Suspensa Phenylethanoid glycosides
DOI	10.3724/SP.J.1096.2011.00323
收录类别	SCI
语种	英语
WOS研究方向	Chemistry
WOS类目	Chemistry, Analytical
WOS记录号	WOS:000290841800006
出版者	ELSEVIER SCIENCE INC
引用统计	被引频次：2[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://119.78.100.204/handle/2XEOYT63/12913
专题	中国科学院计算技术研究所期刊论文_英文
通讯作者	Zeper, Abliz
作者单位	1.Chinese Acad Med Sci, Inst Mat Med, Key Lab Bioact Subst & Resource Utilizat Chinese, Minist Educ, Beijing 100050, Peoples R China 2.Peking Union Med Coll, Beijing 100050, Peoples R China 3.Chinese Acad Sci, Inst Comp Technol, Beijing 100080, Peoples R China
推荐引用方式 GB/T 7714	Liu Ying,He Jiu-Ming,Sun Rui-Xiang,et al. Simultaneous Structural Identification of Constituents in Active Herbal Extract of Forsythia Suspensa Using Nuclear Magnetic Resonance/Liquid Chromatography-Mass Spectrometry Parallel Dynamic Spectroscopy[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2011,39(3):323-329.
APA	Liu Ying.,He Jiu-Ming.,Sun Rui-Xiang.,Liu Chao.,Zhang Rui-Ping.,...&Zeper, Abliz.(2011).Simultaneous Structural Identification of Constituents in Active Herbal Extract of Forsythia Suspensa Using Nuclear Magnetic Resonance/Liquid Chromatography-Mass Spectrometry Parallel Dynamic Spectroscopy.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,39(3),323-329.
MLA	Liu Ying,et al."Simultaneous Structural Identification of Constituents in Active Herbal Extract of Forsythia Suspensa Using Nuclear Magnetic Resonance/Liquid Chromatography-Mass Spectrometry Parallel Dynamic Spectroscopy".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 39.3(2011):323-329.