THREE-DIMENSIONAL RECONSTRUCTION AND VISUALIZATION OF THE MEDIAN NERVE FROM SERIAL TISSUE SECTIONS

doi:10.1002/micr.20646

	THREE-DIMENSIONAL RECONSTRUCTION AND VISUALIZATION OF THE MEDIAN NERVE FROM SERIAL TISSUE SECTIONS
	Sun, Kuo 1; Zhang, Jian 1; Chen, Tongyi 1; Chen, Zhongwei 1; Chen, Zenggan 1; Li, Zhi 1; Li, Hua 2; Hu, Ping 2
	2009
发表期刊	MICROSURGERY
ISSN	0738-1085
卷号	29 期号:7 页码:573-577
摘要	The purpose of this study was to definitively implement the three-dimensional visualization of sensory and motor fascicles in the human median nerve by means of acetylcholinesterase (AChe) histochemical staining and under the assistance of the computer technology. One fresh human median nerve was harvested from a male adult cadaver. The median nerve was fixed at a special holder. Then, the whole holder was embedded and rapidly frozen in the liquid nitrogen. The processed median nerve was then cut coronally every 100 mu m at a 20 mu m thickness along its long axis in a sliding freezing microtome. The total number of sections was 4,650 slices. All sections were stained with the AChe histochemical method. The stained sections were scanned and saved as Joint Photographic Experts Group files. These images with positively and negatively stained sections were acquired to an Intel dual Pentium computer. The Adobe Photoshop CS2 software was used to compare the reference points of images before and after staining. The two-dimensional intraneural microstructure database of median nerve was then acquired. A software of 3D nerve visualization system was developed. With the 3D nerve visualization system, the 3D visualization result of intraneural microstructure of median nerve was created. The findings may provide more accurate and detailed anatomic information for nerve repairs, specifically for the fascicular nerve repairs. The 3D nerve visualization technique may have potential for future studies of topography of peripheral nerve. (C) 2009 Wiley-Liss, Inc. Microsurgery 29:573-577, 2009.
DOI	10.1002/micr.20646
收录类别	SCI
语种	英语
WOS研究方向	Surgery
WOS类目	Surgery
WOS记录号	WOS:000271128500013
出版者	WILEY-LISS
引用统计	被引频次：23[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://119.78.100.204/handle/2XEOYT63/11851
专题	中国科学院计算技术研究所期刊论文_英文
通讯作者	Zhang, Jian
作者单位	1.Fudan Univ, Zhongshan Hosp, Dept Orthopaed, Shanghai 200032, Peoples R China 2.Chinese Acad Sci, Inst Comp Technol, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Sun, Kuo,Zhang, Jian,Chen, Tongyi,et al. THREE-DIMENSIONAL RECONSTRUCTION AND VISUALIZATION OF THE MEDIAN NERVE FROM SERIAL TISSUE SECTIONS[J]. MICROSURGERY,2009,29(7):573-577.
APA	Sun, Kuo.,Zhang, Jian.,Chen, Tongyi.,Chen, Zhongwei.,Chen, Zenggan.,...&Hu, Ping.(2009).THREE-DIMENSIONAL RECONSTRUCTION AND VISUALIZATION OF THE MEDIAN NERVE FROM SERIAL TISSUE SECTIONS.MICROSURGERY,29(7),573-577.
MLA	Sun, Kuo,et al."THREE-DIMENSIONAL RECONSTRUCTION AND VISUALIZATION OF THE MEDIAN NERVE FROM SERIAL TISSUE SECTIONS".MICROSURGERY 29.7(2009):573-577.