A Strategy for Precise and Large Scale Identification of Core Fucosylated Glycoproteins

doi:10.1074/mcp.M800504-MCP200

	A Strategy for Precise and Large Scale Identification of Core Fucosylated Glycoproteins
	Jia, Wei 2; Lu, Zhuang 3; Fu, Yan 4; Wang, Hai-Peng 4; Wang, Le-Heng 4; Chi, Hao 4; Yuan, Zuo-Fei 4; Zheng, Zhao-Bin 1; Song, Li-Na 1; Han, Huan-Huan 1; Liang, Yi-Min 1; Wang, Jing-Lan 1; Cai, Yun 1; Zhang, Yu-Kui 3; Deng, Yu-Lin 3; Ying, Wan-Tao 1; He, Si-Min 4; Qian, Xiao-Hong 1
	2009-05-01
发表期刊	MOLECULAR & CELLULAR PROTEOMICS
ISSN	1535-9476
卷号	8 期号:5 页码:913-923
摘要	Core fucosylation (CF) patterns of some glycoproteins are more sensitive and specific than evaluation of their total respective protein levels for diagnosis of many diseases, such as cancers. Global profiling and quantitative characterization of CF glycoproteins may reveal potent biomarkers for clinical applications. However, current techniques are unable to reveal CF glycoproteins precisely on a large scale. Here we developed a robust strategy that integrates molecular weight cutoff, neutral loss-dependent MS(3), database-independent candidate spectrum filtering, and optimization to effectively identify CF glycoproteins. The rationale for spectrum treatment was innovatively based on computation of the mass distribution in spectra of CF glycopeptides. The efficacy of this strategy was demonstrated by implementation for plasma from healthy subjects and subjects with hepatocellular carcinoma. Over 100 CF glycoproteins and CF sites were identified, and over 10,000 mass spectra of CF glycopeptide were found. The scale of identification results indicates great progress for finding biomarkers with a particular and attractive prospect, and the candidate spectra will be a useful resource for the improvement of database searching methods for glycopeptides. Molecular & Cellular Proteomics 8: 913-923, 2009.
DOI	10.1074/mcp.M800504-MCP200
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[30621063] ; National Natural Science Foundation of China[20735005] ; National Key Program for Basic Research[2006CB910801] ; National Key Program for Basic Research[2002CB713807] ; National Key Program for Basic Research[2004CB518707] ; National Key Program for Basic Research[2007CB914104] ; Hi-Tech Research and Development Program of China[2006AA02A308] ; Hi-Tech Research and Development Program of China[2007AA02Z315] ; Hi-Tech Research and Development Program of China[2008AA02Z309] ; Chinese Academy of Sciences Knowledge Innovation Program[KGGX1-YW-13]
WOS研究方向	Biochemistry & Molecular Biology
WOS类目	Biochemical Research Methods
WOS记录号	WOS:000266116900004
出版者	AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
引用统计	被引频次：84[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://119.78.100.204/handle/2XEOYT63/11751
专题	中国科学院计算技术研究所期刊论文_英文
通讯作者	Ying, Wan-Tao
作者单位	1.Beijing Inst Radiat Med, Beijing Proteome Res Ctr, State Key Lab Prote, Beijing 102206, Peoples R China 2.Chinese Acad Sci, Inst Biophys, Beijing 100101, Peoples R China 3.Beijing Inst Technol, Beijing 100081, Peoples R China 4.Chinese Acad Sci, Inst Comp Technol, Beijing 100190, Peoples R China
推荐引用方式 GB/T 7714	Jia, Wei,Lu, Zhuang,Fu, Yan,et al. A Strategy for Precise and Large Scale Identification of Core Fucosylated Glycoproteins[J]. MOLECULAR & CELLULAR PROTEOMICS,2009,8(5):913-923.
APA	Jia, Wei.,Lu, Zhuang.,Fu, Yan.,Wang, Hai-Peng.,Wang, Le-Heng.,...&Qian, Xiao-Hong.(2009).A Strategy for Precise and Large Scale Identification of Core Fucosylated Glycoproteins.MOLECULAR & CELLULAR PROTEOMICS,8(5),913-923.
MLA	Jia, Wei,et al."A Strategy for Precise and Large Scale Identification of Core Fucosylated Glycoproteins".MOLECULAR & CELLULAR PROTEOMICS 8.5(2009):913-923.