MicroRNA-encoding long non-coding RNAs

doi:10.1186/1471-2164-9-236

	MicroRNA-encoding long non-coding RNAs
	He, Shunmin 2,3,4; Su, Hua 2,3,4; Liu, Changning 1,4; Skogerbo, Geir 2,3; He, Housheng 2,3; He, Dandan 2,3; Zhu, Xiaopeng 2,3; Liu, Tao 2,3; Zhao, Yi 1; Chen, Runsheng 2,3
	2008-05-21
发表期刊	BMC GENOMICS
ISSN	1471-2164
卷号	9 页码:11
摘要	Background: Recent analysis of the mouse transcriptional data has revealed the existence of similar to 34,000 messenger-like non-coding RNAs (ml-ncRNAs). Whereas the functional properties of these ml-ncRNAs are beginning to be unravelled, no functional information is available for the large majority of these transcripts. Results: A few ml-ncRNA have been shown to have genomic loci that overlap with microRNA loci, leading us to suspect that a fraction of ml-ncRNA may encode microRNAs. We therefore developed an algorithm (PriMir) for specifically detecting potential microRNA-encoding transcripts in the entire set of 34,030 mouse full-length ml-ncRNAs. In combination with mouse-rat sequence conservation, this algorithm detected 97 ( 80 of them were novel) strong miRNA-encoding candidates, and for 52 of these we obtained experimental evidence for the existence of their corresponding mature microRNA by microarray and stem-loop RT-PCR. Sequence analysis of the microRNA-encoding RNAs revealed an internal motif, whose presence correlates strongly (R(2) = 0.9, P-value = 2.2 x 10(-16)) with the occurrence of stem-loops with characteristics of known pre-miRNAs, indicating the presence of a larger number microRNA-encoding RNAs (from 300 up to 800) in the ml-ncRNAs population. Conclusion: Our work highlights a unique group of ml-ncRNAs and offers clues to their functions.
DOI	10.1186/1471-2164-9-236
收录类别	SCI
语种	英语
WOS研究方向	Biotechnology & Applied Microbiology ; Genetics & Heredity
WOS类目	Biotechnology & Applied Microbiology ; Genetics & Heredity
WOS记录号	WOS:000256400500001
出版者	BIOMED CENTRAL LTD
引用统计	被引频次：49[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://119.78.100.204/handle/2XEOYT63/11148
专题	中国科学院计算技术研究所期刊论文_英文
通讯作者	Chen, Runsheng
作者单位	1.Chinese Acad Sci, Inst Comp Technol, Bioinformat Res Grp, Beijing, Peoples R China 2.Chinese Acad Sci, Inst Biophys, Bioinformat Lab, Beijing 100080, Peoples R China 3.Chinese Acad Sci, Inst Biophys, Natl Lab Biomacromol, Beijing 100080, Peoples R China 4.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China
推荐引用方式 GB/T 7714	He, Shunmin,Su, Hua,Liu, Changning,et al. MicroRNA-encoding long non-coding RNAs[J]. BMC GENOMICS,2008,9:11.
APA	He, Shunmin.,Su, Hua.,Liu, Changning.,Skogerbo, Geir.,He, Housheng.,...&Chen, Runsheng.(2008).MicroRNA-encoding long non-coding RNAs.BMC GENOMICS,9,11.
MLA	He, Shunmin,et al."MicroRNA-encoding long non-coding RNAs".BMC GENOMICS 9(2008):11.