In vivo analysis of Caenorhabditis elegans noncoding RNA promoter motifs

doi:10.1186/1471-2199-9-71

	In vivo analysis of Caenorhabditis elegans noncoding RNA promoter motifs
	Li, Tiantian 1,4,6; He, Housheng 1,4,6; Wang, Yunfei 1,4,6; Zheng, Haixia 1,5,6; Skogerbo, Geir 1,6; Chen, Runsheng 1,2,3,6
	2008-08-05
发表期刊	BMC MOLECULAR BIOLOGY
ISSN	1471-2199
卷号	9 页码:14
摘要	Background: Noncoding RNAs (ncRNAs) play important roles in a variety of cellular processes. Characterizing the transcriptional activity of ncRNA promoters is therefore a critical step toward understanding the complex cellular roles of ncRNAs. Results: Here we present an in vivo transcriptional analysis of three C. elegans ncRNA upstream motifs (UM1-3). Transcriptional activity of all three motifs has been demonstrated, and mutational analysis revealed differential contributions of different parts of each motif. We showed that upstream motif 1 (UM1) can drive the expression of green fluorescent protein (GFP), and utilized this for detailed analysis of temporal and spatial expression patterns of 5 SL2 RNAs. Upstream motifs 2 and 3 do not drive GFP expression, and termination at consecutive T runs suggests transcription by RNA polymerase III. The UM2 sequence resembles the tRNA promoter, and is actually embedded within its own short-lived, primary transcript. This is a structure which is also found at a few plant and yeast loci, and may indicate an evolutionarily very old dicistronic transcription pattern in which a tRNA serves as a promoter for an adjacent snoRNA. Conclusion: The study has demonstrated that the three upstream motifs UM1-3 have promoter activity. The UM1 sequence can drive expression of GFP, which allows for the use of UM1:: GFP fusion constructs to study temporal-spatial expression patterns of UM1 ncRNA loci. The UM1 loci appear to act in concert with other upstream sequences, whereas the transcriptional activities of the UM2 and UM3 are confined to the motifs themselves.
DOI	10.1186/1471-2199-9-71
收录类别	SCI
语种	英语
资助项目	NIH National Center for Research Resources ; National Sciences Foundation of China[30630040] ; National Key Basic Research & Development Program 973[2002CB713805] ; National Key Basic Research & Development Program 973[2003CB715907]
WOS研究方向	Biochemistry & Molecular Biology
WOS类目	Biochemistry & Molecular Biology
WOS记录号	WOS:000258792700001
出版者	BIOMED CENTRAL LTD
引用统计	被引频次：13[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://119.78.100.204/handle/2XEOYT63/11126
专题	中国科学院计算技术研究所期刊论文_英文
通讯作者	Skogerbo, Geir
作者单位	1.Chinese Acad Sci, Inst Psychol, Bioinformat Lab, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Inst Comp Technol, Bioinformat Res Grp, Key Lab Intelligent Informat Proc, Beijing 100080, Peoples R China 3.Chinese Natl Human Genome Ctr, Beijing 100176, Peoples R China 4.Chinese Acad Sci, Grad Sch, Beijing 100080, Peoples R China 5.Shanxi Agr Univ, Taigu 030801, Shanxi, Peoples R China 6.Chinese Acad Sci, Inst Psychol, Natl Lab Biomacromol, Beijing 100101, Peoples R China
推荐引用方式 GB/T 7714	Li, Tiantian,He, Housheng,Wang, Yunfei,et al. In vivo analysis of Caenorhabditis elegans noncoding RNA promoter motifs[J]. BMC MOLECULAR BIOLOGY,2008,9:14.
APA	Li, Tiantian,He, Housheng,Wang, Yunfei,Zheng, Haixia,Skogerbo, Geir,&Chen, Runsheng.(2008).In vivo analysis of Caenorhabditis elegans noncoding RNA promoter motifs.BMC MOLECULAR BIOLOGY,9,14.
MLA	Li, Tiantian,et al."In vivo analysis of Caenorhabditis elegans noncoding RNA promoter motifs".BMC MOLECULAR BIOLOGY 9(2008):14.