Systematic identification of non-coding RNA 2,2,7-trimethylguanosine cap structures in Caenorhabditis elegans

doi:10.1186/1471-2199-8-86

	Systematic identification of non-coding RNA 2,2,7-trimethylguanosine cap structures in Caenorhabditis elegans
	Jia, Dong 1,2; Cai, Lun 3,4; He, Housheng 1,4; Skogerbo, Geir 1; Li, Tiantian 1,4; Aftab, Muhammad Nauman 1,4; Chen, Runsheng 1
	2007-09-29
发表期刊	BMC MOLECULAR BIOLOGY
ISSN	1471-2199
卷号	8 页码:9
摘要	Background: The 2,2,7-trimethylguanosine (TMG) cap structure is an important functional characteristic of ncRNAs with critical cellular roles, such as some snRNAs. Here we used immunoprecipitation with both K121 and R1131 anti-TMG antibodies to systematically identify the TMG cap structures for all presently characterized ncRNAs in C. elegans. Results: The two anti-TMG antibodies precipitated a similar group of the C. elegans ncRNAs. All snRNAs known to have a TMG cap structure were found in the precipitate, indicating that our identification system was efficient. Other ncRNA families related to splicing, such as SL RNAs and Sm Y RNAs, were also found in the precipitate, as were 7 C/D box snoRNAs. Further analysis showed that the SL RNAs and the Sm Y RNAs shared a very similar Sm binding site element ( AAU(4)-(5)GGA), which sequence composition differed somewhat from those of other U snRNAs. There were also 16 ncRNAs without an Sm binding site element in the precipitate, suggesting that for these ncRNAs, TMG formation may occur independently of Sm proteins. Conclusion: Our results showed that most ncRNAs predicted to be transcribed by RNA polymerase II had a TMG cap, while those predicted to be transcribed by RNA plymerase III or located in introns did not have a TMG cap structure. Compared to ncRNAs without a TMG cap, TMG-capped ncRNAs tended to have higher expression levels. Five functionally non-annotated ncRNAs also have a TMG cap structure, which might be helpful for identifying the cellular roles of these ncRNAs.
DOI	10.1186/1471-2199-8-86
收录类别	SCI
语种	英语
WOS研究方向	Biochemistry & Molecular Biology
WOS类目	Biochemistry & Molecular Biology
WOS记录号	WOS:000252379900001
出版者	BIOMED CENTRAL LTD
引用统计	被引频次：11[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://119.78.100.204/handle/2XEOYT63/10895
专题	中国科学院计算技术研究所期刊论文_英文
通讯作者	Chen, Runsheng
作者单位	1.Chinese Acad Sci, Inst Biophys, Bioinformat Lab, Beijing 100101, Peoples R China 2.Shanxi Agr Univ, Taigu 030801, Peoples R China 3.Chinese Acad Sci, Inst Comp Technol, Key Lab Intelligent Informat Proc, Beijing 100080, Peoples R China 4.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
推荐引用方式 GB/T 7714	Jia, Dong,Cai, Lun,He, Housheng,et al. Systematic identification of non-coding RNA 2,2,7-trimethylguanosine cap structures in Caenorhabditis elegans[J]. BMC MOLECULAR BIOLOGY,2007,8:9.
APA	Jia, Dong.,Cai, Lun.,He, Housheng.,Skogerbo, Geir.,Li, Tiantian.,...&Chen, Runsheng.(2007).Systematic identification of non-coding RNA 2,2,7-trimethylguanosine cap structures in Caenorhabditis elegans.BMC MOLECULAR BIOLOGY,8,9.
MLA	Jia, Dong,et al."Systematic identification of non-coding RNA 2,2,7-trimethylguanosine cap structures in Caenorhabditis elegans".BMC MOLECULAR BIOLOGY 8(2007):9.